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首页> 外文期刊>Fisheries Science >Effect of gamma-aminobutyric acid and porcine growth hormone on survival of the euryhaline rotifers Brachionus plicatilis sp. complex preserved at low temperature
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Effect of gamma-aminobutyric acid and porcine growth hormone on survival of the euryhaline rotifers Brachionus plicatilis sp. complex preserved at low temperature

机译：γ-氨基丁酸和猪生长激素对轮虫Brachionus plicatilis sp。存活的影响。低温保存的复合物

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摘要

Gamma-aminobutyric acid (GABA) at 50 μg/ml and porcine growth hormone (GH) at 0.025 IU/ml were tested to see whether these chemicals would reduce the stress experienced by euryhaline rotifers Brachionus plicatilis species complex (L-, S- and SS-morphotypes) during low temperature (4–12°C) storage. Rotifers cultured at 25°C were transferred to 4–12°C for 10–30 days and transferred back to 25°C for recovery. GABA or GH were added to the rotifers at three different time points: 6 h before transfer from 25°C to low temperature (6h−), on day 7 after preservation at low temperature (7d+) and on the first day of recovery. For L-type rotifers, the GH treatment before the transfer to 4°C for 30 days was effective for better survival, while the GABA treatment was most effective for the S-type preserved at 10°C for 14 days. For the SS-type, the chemical treatments were not effective when the rotifers were preserved at 12°C for 14 days. After low temperature preservation, GABA treatments with the S- and SS-type rotifers just after their transfer to 25°C induced a relatively faster recovery of the rotifer population.
机译：测试了50μg/ ml的γ-氨基丁酸（GABA）和0.025 IU / ml的猪生长激素（GH），以查看这些化学物质是否能减轻轮虫Brachionus plicatilis种复合物（L-，S-和SS型）在低温（4–12°C）储存期间。将在25°C下培养的轮虫转移到4–12°C放置10–30天，然后转移回25°C以进行恢复。在三个不同的时间点将GABA或GH添加到轮虫中：从25°C转移到低温前6小时（6h-），低温保存后第7天（7d +）和恢复的第一天。对于L型轮虫，在转移至4°C 30天之前进行GH处理对于更好的存活是有效的，而GABA处理对于在10°C下保存14天的S型最为有效。对于SS型，当轮虫在12°C下保存14天时，化学处理无效。低温保存后，将S型和SS型轮虫转移到25°C后立即对其进行GABA处理，导致轮虫种群的恢复相对较快。

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