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Otolith microstructure of brown sole Pseudopleuronectes herzensteini: validation of daily ring formation and the occurrence of microstructure denoting metamorphosis

机译:棕色唯一假单肺假单胞菌herzensteini的耳石微结构:日常环形成的验证和表明变态的微结构的发生

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摘要

Brown sole Pseudopleuronectes herzensteini larvae and juveniles were reared to validate daily otolith ring formation. At 15°C, a check (a distinct ring) formed on the sagittae and lapilli at 6 days after hatching, and clear increments regularly formed outside the check. For both otoliths, the relationship between the number of days after hatching and number of increments was linear, and the slope of the line was approximately 1; therefore, daily formation was validated. At 12°C, the check formed on the lapillus 8 days after hatching. Accessory primordia (AP) began forming on the sagittae of metamorphosing larvae, and the shape of the sagittae became complicated. AP were not formed on the lapillus; concentric rings were formed throughout larval and juvenile stages. Wide and obscure increments formed on the lapilli during metamorphosis (metamorphosing zone, MZ). Based on MZ, concentric rings that have formed on the lapilli of juveniles can be separated into larval and juvenile rings. The morphs of large juveniles’ lapilli were bilaterally asymmetric, and the blind-side lapilli were most suitable for otolith microstructure analysis. This study provides fundamental information for otolith microstructure analysis in wild brown sole.
机译:饲养棕色唯一的假单囊假单胞菌herzensteini幼虫和幼体以验证每日耳石环的形成。在15°C下,孵化后第6天,在人马座和青虫上形成了一个支票(一个明显的环),并在支票外面定期形成明显的增量。对于这两种耳石,孵化后的天数与增量数之间的关系是线性的,直线的斜率约为1;因此,每日形成被验证。孵化8天后,在12°C时,在支原体上形成了检查。附件原基(AP)开始在变态幼虫的矢状面上形成,并且矢状的形状变得复杂。 AP没有形成在lapillus上;在幼虫和幼年期形成同心环。在变态过程中(变形区,MZ),在羊膜上形成了宽而模糊的增量。基于MZ,可以将在幼虫的小环上形成的同心环分为幼虫环和幼体环。大型幼虫的蓝宝石的形态是两侧不对称的,而盲侧的蓝宝石最适合于耳石的显微结构分析。这项研究为野生棕色鞋底耳石微结构分析提供了基础信息。

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