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Immunoreactive changes in pituitary FSH and LH cells during seasonal reproductive and spawning cycles of female chub mackerel Scomber japonicus

机译:鱼鲭鱼季节性繁殖和产卵周期中垂体FSH和LH细胞的免疫反应性变化

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摘要

The physiological functions of pituitary gonadotropins (GtHs) are well established in higher vertebrates, whereas those in teleosts are still poorly understood. To describe the role of GtHs during gonadal development of female chub mackerel Scomber japonicus, changes in follicle-stimulating hormone (FSH) and luteinizing hormone (LH) cells were investigated immunohistochemically during the seasonal reproductive and spawning cycles. FSH and LH cells were identified in the different cell types of the proximal pars distalis (PPD); FSH cells were located in the central PPD, whereas LH cells were localized along the border of the pars intermedia. To examine changes in FSH and LH cells, the percentage of FSH or LH cell-occupying area in the PPD was evaluated and represented as FSHβ-immunoreactive (ir) or LHβ-ir levels, respectively. FSHβ-ir levels increased significantly from immature to the completion of vitellogenesis, whereas LHβ-ir levels were maintained at high levels from early vitellogenesis to post-spawning. During the spawning cycle, which consisted of four stages from just after spawning to the next oocyte maturation, both FSHβ-ir and LHβ-ir levels showed no significant changes among different stages; however, LHβ-ir levels remained relatively high, and FSHβ-ir levels were constantly low. These results suggest that both FSH and LH may be involved in vitellogenesis and LH may act at final oocyte maturation in female chub mackerel, although the role of FSH during the spawning cycle is still unclear.
机译:垂体促性腺激素(GtHs)的生理功能在高等脊椎动物中已得到充分确立,而硬骨鱼的这些功能仍知之甚少。为了描述GtHs在雌性chu鱼鲭鱼的性腺发育中的作用,在季节性繁殖和产卵周期中,通过免疫组织化学方法研究了卵泡刺激素(FSH)和黄体生成素(LH)细胞的变化。 FSH和LH细胞是在远端近端(PPD)的不同细胞类型中鉴定的; FSH细胞位于中央PPD,而LH细胞则位于pars intermedia的边界。为了检查FSH和LH细胞的变化,评估了PPD中FSH或LH细胞占用面积的百分比,并分别表示为FSHβ免疫反应(ir)或LHβ-ir水平。从未成熟到卵黄形成完成,FSHβ-ir水平显着增加,而从卵黄早期形成到产卵后,LHβ-ir水平保持较高水平。在产卵周期中,从产卵到下一个卵母细胞成熟包括四个阶段,FSHβ-ir和LHβ-ir的水平在不同阶段之间没有显着变化。但是,LHβ-ir水平仍然相对较高,而FSHβ-ir水平却一直较低。这些结果表明,FSH和LH都可能参与卵黄形成,而LH可能在雌性鱼的最终卵母细胞成熟中起作用,尽管FSH在产卵周期中的作用仍不清楚。

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