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Genetic identification and morphology of naturally spawned eggs of the Japanese eel Anguilla japonica collected in the western North Pacific

机译:北太平洋西部采集的日本鳗An天然产卵的遗传鉴定和形态学

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Eggs of the Japanese eel Anguilla japonica collected in the western North Pacific were identified by onboard species-specific polymerase chain reaction (PCR) and DNA nucleotide sequencing after the cruise. Fish eggs of various species were collected by large plankton net tows at 12 stations along the southern part of the West Mariana Ridge on 19–25 May 2009. A total of 43 fish eggs were distinguished morphologically as possibly being of A. japonica. Thirty-one of those were analyzed by PCR, which included 15 eggs collected at 12°50–55′N, 141°15–20′E (in 5 tows) that showed positive results. The 16S ribosomal RNA (rRNA) gene sequences of eggs determined after the cruise indicated that 31 A. japonica eggs had been collected. The remaining eggs were of mesopelagic eel species (Serrivomeridae and Derichthyidae), or unidentified species. The morphological characteristics of the A. japonica eggs were consistent with those of artificially spawned eggs, except they had a slightly larger diameter. The egg diameter range did not overlap with those of mesopelagic eels of the Serrivomeridae, which often spawn in the same area as A. japonica. These results suggest that egg diameter and embryo shape can be used to morphologically identify naturally spawned A. japonica eggs.
机译:在航行后,通过船上物种特异性聚合酶链反应(PCR)和DNA核苷酸测序,鉴定了在北太平洋西部采集的日本鳗e的卵。 2009年5月19日至25日,在西马里亚纳海岭南部的12个站点上,通过大型浮游生物网状丝束收集了各种鱼卵。在形态学上,共有43种鱼卵被辨别为粳稻。其中31个通过PCR进行了分析,其中包括在12°50-55'N,141°15-20'E(5根丝束)中收集的15个卵,均显示阳性结果。航行后确定的鸡蛋的16S核糖体RNA(rRNA)基因序列表明已收集到31个粳稻鸡蛋。剩下的卵是中生鳗鱼种(Serrivomeridae和Derichthyidae)或未鉴定的种。粳稻卵的形态特征与人工产卵的形态特征一致,只是直径略大。卵直径范围与锯齿科的近中鳗不重叠,后者通常在与日本粳稻相同的区域产卵。这些结果表明,卵直径和胚形可用于从形态上鉴定天然产生的粳稻卵。

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