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首页> 外文期刊>Fisheries Science >Molecular characterization and expression analysis of heat shock proteins 40, 70 and 90 from kuruma shrimp Marsupenaeus japonicus
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Molecular characterization and expression analysis of heat shock proteins 40, 70 and 90 from kuruma shrimp Marsupenaeus japonicus

机译：极地虾仁对虾热激蛋白40、70和90的分子表征和表达分析

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摘要

Heat shock proteins (HSPs) are proteins that are expressed more strongly when the cells are exposed to physiological and stressful conditions. In this study, the full-length cDNAs of heat shock proteins 40 (MjHSP40), 70 (MjHSP70) and 90 (MjHSP90) were cloned from kuruma shrimp Marsupenaeus japonicus. The open reading frames (ORFs) of the cDNA clones have lengths of 1,191, 1,959 and 2,172 bp and encode 396, 652 and 723 amino acid residues, respectively. The predicted MjHSP40 amino acid sequence contains a J domain, a glycine/phenylalanine-rich region, and a central domain containing four repeats of a CxxCxGxG motif, indicating that it is a type I HSP40 homolog. The signature sequences of the HSP70 and HSP90 gene families are conserved in the MjHSP70 and MjHSP90 amino acid sequences. The deduced amino acid sequences of MjHSP70 and MjHSP90 share high identity with previously reported shrimp HSP70s and HSP90s, respectively. The expression of MjHSP90 mRNA increased at 32°C. Additionally, the expressions of MjHSP40, MjHSP70 and MjHSP90 mRNAs increased in defense-related tissues (i.e., hemocytes and lymphoid organ) when the shrimp were challenged with white spot syndrome virus.
机译：热休克蛋白（HSP）是当细胞暴露于生理和压力条件下时表达更强的蛋白。在这项研究中，从库鲁马虾Marsupenaeus japonicus克隆了热激蛋白40（MjHSP40），70（MjHSP70）和90（MjHSP90）的全长cDNA。 cDNA克隆的开放阅读框（ORF）的长度为1,191、1,959和2,172 bp，分别编码396、652和723个氨基酸残基。预测的MjHSP40氨基酸序列包含一个J结构域，一个富含甘氨酸/苯丙氨酸的区域以及一个包含CxxCxGxG基序的四个重复序列的中央结构域，表明它是I型HSP40同源物。 HSP70和HSP90基因家族的签名序列在MjHSP70和MjHSP90氨基酸序列中是保守的。推导的MjHSP70和MjHSP90的氨基酸序列分别与先前报道的虾HSP70和HSP90具有高度同一性。在32℃下，MjHSP90 mRNA的表达增加。此外，当虾受到白斑综合症病毒攻击时，在防御相关组织（即血细胞和淋巴器官）中，MjHSP40，MjHSP70和MjHSP90 mRNA的表达增加。

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