• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Fisheries Science >Molecular cloning and tissue transcriptional analysis of a novel gene encoding proteasome activator PA28-α from common carp (Cyprinus carpio L.)
【24h】

Molecular cloning and tissue transcriptional analysis of a novel gene encoding proteasome activator PA28-α from common carp (Cyprinus carpio L.)

机译：鲤（Cyprinus carpio L.）蛋白酶体激活因子PA28-α编码新基因的分子克隆和组织转录分析

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

To identify the homolog of the PA28-α subunit, a leukocyte cDNA library of common carp was screened and the full-length cDNA sequence of the PSME1 gene coding for the PA28-α was obtained. It encompasses 1097 nucleotides (nt), with an open reading frame encoding 249 amino acids. The deduced protein sequence exhibits identities of 92, 78, 78, 77, 75, 56, 54, 53 and 54% with zebrafish, Atlantic salmon, northern pike, rainbow trout, rainbow smelt, mouse, pig, cattle and human PA28-α subunits, respectively. Multiple sequence alignment of the PA28-α protein of common carp and other known PA28 subunits indicates that it contains a PA28-α subunit-specific insert corresponding to the KEKE motif of the known PA28-α (Region B), a characteristic proline-rich motif (Region A), a potential protein kinase C recognition site (Region D), a conserved activation loop (Region C), and a highly homologous C-terminal region (Region E). Phylogenetic analysis reveals that the PA28-γ subunit is related to the presumed ancestor of the PA28-α and PA28-β subunits. In addition, the genomic DNA obtained by PCR is composed of eleven exons and ten introns with 3583 nt, typical of the PA28-α gene organization. Compared with the other known PA28-α genomic DNA, in spite of structural conservation through evolution, the fish PA28-α genes showed a much greater sequence divergence than their counterparts among mammals. Tissue transcriptional analysis of carp PA28-α indicated that it was visibly enhanced in tissues associated with immunity (gill, spleen, kidney and liver), and weakly transcribed in muscle, brain and heart.
机译：为了鉴定PA28-α亚基的同源物，筛选了鲤鱼的白细胞cDNA文库，并获得了编码PA28-α的PSME1基因的全长cDNA序列。它包含1097个核苷酸（nt），带有一个开放阅读框，编码249个氨基酸。推导的蛋白质序列与斑马鱼，大西洋鲑鱼，北梭鱼，虹鳟鱼，虹鳟鱼，小鼠，猪，牛和人PA28-α的同一性分别为92、78、78、77、75、56、54、53和54％亚基。鲤鱼和其他已知的PA28亚基的PA28-α蛋白的多序列比对表明它含有一个PA28-α亚基特异性插入物，对应于已知PA28-α（区域B）的KEKE基序（富含脯氨酸的特征）基序（区域A），潜在的蛋白激酶C识别位点（区域D），保守的激活环（区域C）和高度同源的C端区域（区域E）。系统发育分析表明，PA28-γ亚基与PA28-α和PA28-β亚基的祖先有关。另外，通过PCR获得的基因组DNA由11个外显子和10个具有3583nt的内含子组成，这是PA28-α基因组织的典型特征。与其他已知的PA28-α基因组DNA相比，尽管通过进化进行了结构保护，但鱼类PA28-α基因的序列差异远大于哺乳动物中的PA28-α基因。鲤鱼PA28-α的组织转录分析表明，鲤鱼PA28-α在与免疫相关的组织（g，脾脏，肾脏和肝脏）中明显增强，在肌肉，脑和心脏中的转录较弱。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号