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首页> 外文期刊>Food additives & contaminants >Natural incidence of Fusarium species and fumonisins B_1 and B_2 associated with maize kernels from nine provinces in China in 2012
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Natural incidence of Fusarium species and fumonisins B_1 and B_2 associated with maize kernels from nine provinces in China in 2012

机译：2012年中国9个省玉米相关镰刀菌种和伏马菌素B_1和B_2的自然发生率

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摘要

Fusarium species, which can produce mycotoxins, are the predominant pathogens causing maize ear rot, a disease that results in severe economic losses and serves as a potential health risk for humans and animals. A survey was conducted in 2012 to investigate the contamination of maize by Fusarium species and fumonisins B_1 and B_2. A total of 250 maize samples were randomly collected from nine provinces (Hebei, Shanxi, Inner Mongolia, Yunnan, Sichuan, Guizhou, Heilongjiang, Liaoning and Ningxia) in China. Fusarium species were isolated and identified using morphological (electron microscope) and molecular methods (polymerase chain reaction (PCR) and sequencing). Fumonisins B_1 and B_2 were analysed using high-performance liquid chromatography with fluorescence detection (HPLC-FLD) with OPA (2-Mercaptoethanol, o-phthaldialdehyde) post-column derivatisation. A total of 2321 Fusarium isolates (20.7%) were obtained from all the samples. These isolates included nine Fusarium species, namely, F. graminearum, F. verticillioides, F. subglutinans, F. proliferatum, F. temperatum, F. oxysporum, F. equiseti, F. meridionale and F. chlamydosporum. The incidence of occurrence of Fusarium species in Guizhou was the highest, while in Inner Mongolia it was the lowest. F. verticillioides was the dominant species of maize ear rot in Liaoning, Sichuan, Hebei and Ningxia. F. graminearum was the dominant species in Yunnan, Guizhou and Shanxi. F. subglutinans was the dominant species in Heilongjiang. F. verticillioides and F. graminearum percentages were the same in Inner Mongolia. The incidence of fumonisins in Liaoning was high (up to 81.0%) and in Heilongjiang low (up to 10.3%). Except Shanxi, more than 50% of maize samples from other provinces were contaminated with fumonisins, with concentrations less than 500 ng g~(-1). About 33% of maize samples from Yunnan were contaminated with high levels of fumonisins, and average of fumonisin levels were 5191 ng g~(-1). Fusarium species causing maize ear rot in different areas in China were highly diverse and such areas with exposure to high levels of fumonisin contamination have a potential health risk for human and animals.
机译：镰刀菌可能产生霉菌毒素，是导致玉米腐烂的主要病原体，这种疾病导致严重的经济损失，并可能对人类和动物造成健康危害。 2012年进行了一项调查，调查了镰刀菌属物种和伏马菌素B_1和B_2对玉米的污染。从中国9个省（河北，山西，内蒙古，云南，四川，贵州，黑龙江，辽宁和宁夏）随机抽取了250个玉米样品。使用形态学（电子显微镜）和分子方法（聚合酶链反应（PCR）和测序）分离和鉴定镰刀菌。柱后衍生化使用带有OPA（2-巯基乙醇，邻苯二甲醛）的荧光检测（HPLC-FLD）的高效液相色谱法分析伏马菌素B_1和B_2。从所有样品中总共获得了2321株镰刀菌分离株（20.7％）。这些分离物包括九种镰刀菌属，即禾谷镰刀菌，网状镰刀菌，谷胶镰刀菌，pro.proliferatum，F.tempatum，oxysporum，F。equiseti，meridionale和F. chlamydosporum。贵州镰刀菌种的发生率最高，而内蒙古最低。黄萎病菌是辽宁，四川，河北和宁夏的玉米穗腐病的优势种。禾谷镰刀菌是云南，贵州和山西的优势种。谷粉镰刀菌是黑龙江省的优势种。内蒙古F. verticillioides和F. graminearum的百分比相同。在辽宁，伏马毒素的发病率较高（高达81.0％），而在黑龙江则较低（高达10.3％）。除山西外，其他省份的玉米样品中有超过50％的烟曲霉毒素污染浓度低于500 ng g〜（-1）。云南省约33％的玉米样品被高水平的伏马菌素污染，伏马菌素的平均含量为5191 ng g〜（-1）。在中国不同地区，造成玉米穗腐病的镰刀菌种类非常多样，而且此类地区受到伏马菌素污染程度很高，对人类和动物都有潜在的健康风险。

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  • 来源
    《Food additives & contaminants》 |2015年第4期|503-511|共9页
  • 作者

    Meng Fu; Renjie Li; Congcong Guo; Minhao Pang; Yingchao Liu; Jingao Dong;

  • 作者单位

    College of Plant Protection, Agricultural University of Hebei, Baoding, China,Laboratory of Mycotoxin and Molecular Plant Pathology, Agricultural University of Hebei, Baoding, China;

    College of Plant Protection, Agricultural University of Hebei, Baoding, China,Laboratory of Mycotoxin and Molecular Plant Pathology, Agricultural University of Hebei, Baoding, China;

    College of Plant Protection, Agricultural University of Hebei, Baoding, China,Laboratory of Mycotoxin and Molecular Plant Pathology, Agricultural University of Hebei, Baoding, China;

    College of Plant Protection, Agricultural University of Hebei, Baoding, China;

    College of Plant Protection, Agricultural University of Hebei, Baoding, China,Laboratory of Mycotoxin and Molecular Plant Pathology, Agricultural University of Hebei, Baoding, China;

    Laboratory of Mycotoxin and Molecular Plant Pathology, Agricultural University of Hebei, Baoding, China;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    maize; Fusarium species; morphological; molecular method; fumonisins;

    机译：玉米;镰刀菌属;形态分子方法伏马菌素;

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