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首页> 外文期刊>Food additives & contaminants >Impact of non-selective fungicides on the growth and production of ochratoxin A by Aspergillus ochraceus and A. carbonarius in barley-based medium
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Impact of non-selective fungicides on the growth and production of ochratoxin A by Aspergillus ochraceus and A. carbonarius in barley-based medium

机译:非选择性杀菌剂对och米曲霉和碳曲霉在大麦基培养基中medium曲霉毒素A生长和生产的影响

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摘要

The aim of this study was to assess the influence of the non-selective fungicides mancozeb, copper oxychloride, and sulfur on the growth and capability for producing ochratoxin A (OTA) of ochratoxigenic isolates of Aspergillus carbonarius and A. ochraceus in barley-based medium. Lag phases and growth rates were determined for each fungicide at different doses, at 15℃ and 25℃ and at 0.97 a_w. Mancozeb at 40 mgl~(-1) inhibited fungal growth and provided lag phases >24 days at 10-20mgl~' and 15℃. OTA was observed only at 25℃ and doses < 10mgl~(-1). At 15℃, copper oxychloride proved inhibitory at 800mgl~(-1), while at 25℃ growth was not delayed and only high doses decreased OTA levels. Sulfur was inhibitory or provided large lag phases at 5-8 gl~(-1) (at 15℃) while at 25℃ growth took place even at 8 gl~(-1), although OTA levels were low or undetectable. The antifungal activity decreased in the order mancozeb > copper oxychloride > sulfur, and was lower at 25℃ than at 15℃. OTA accumulation was affected by the type of fungicide, dose, temperature and time. The efficacy of these fungicides on the growth of A. carbonarius and A. ochraceus and OTA production in barley-based medium is assessed for the first time.
机译:这项研究的目的是评估在基于大麦的培养基中,非选择性杀真菌剂mancozeb,氯氧化铜和硫对carbon曲霉和ari曲霉的产毒毒素菌株的growth曲毒素A(OTA)的生长和生产to曲毒素A(OTA)的影响。 。在15℃和25℃以及0.97 a_w下,以不同的剂量测定每种杀真菌剂的滞后期和生长速率。 40 mgl〜(-1)的Mancozeb抑制真菌生长,在10-20mgl〜和15℃下提供> 24天的滞后期。 OTA仅在25℃下观察到,剂量<10mgl〜(-1)。在15℃时,氯化氧铜被证明对800mgl〜(-1)有抑制作用,而在25℃时,生长没有延迟,只有高剂量时OTA水平降低。尽管OTA含量较低或无法检测到,但硫在5-8 gl〜(-1)(15℃)时具有抑制作用或提供较大的滞后相,而在25℃甚至在8 gl〜(-1)时也有生长。抑菌活性以代森锰锌>氯氧化铜>硫的顺序降低,在25℃时比在15℃时低。 OTA积累受杀菌剂类型,剂量,温度和时间的影响。首次评估了这些杀菌剂对基于大麦的培养基中碳曲霉和growth曲霉的生长以及OTA产生的功效。

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  • 来源
    《Food additives & contaminants》 |2011年第1期|p.86-97|共12页
  • 作者单位

    Dep. de Microbiologia y Ecologia, Universitat de Valencia, Dr. Moliner 50, E-46100 Burjassot, Valencia, Spain;

    Dep. de Microbiologia y Ecologia, Universitat de Valencia, Dr. Moliner 50, E-46100 Burjassot, Valencia, Spain;

    Dep. de Microbiologia y Ecologia, Universitat de Valencia, Dr. Moliner 50, E-46100 Burjassot, Valencia, Spain;

    Dep. de Microbiologia y Ecologia, Universitat de Valencia, Dr. Moliner 50, E-46100 Burjassot, Valencia, Spain;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    mycology; microbiology; high-performance liquid chromatography (HPLC); mycotoxins; ochratoxin a; cereals;

    机译:真菌学微生物学;高效液相色谱(HPLC);霉菌毒素;曲毒素a;谷物;

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