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Evaluation of commercially available ELISA test kits for the detection of clenbuterol and other β2-agonists

机译:评估用于检测盐酸克伦特罗和其他β2-激动剂的市售ELISA测试试剂盒

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摘要

This article presents the results of a comparative study of nine commercially available ELISA test kits for clenbuterol and other β2-agonists currently being used in veterinary drug residue control of live and slaughtered animals in the European Union (EU). By determining measuring ranges, B/B_0-50% values, inter- and intra-assay variations and cross-reactions as well as limits of detection (LOD) and recoveries for clenbuterol in bovine urine it could be demonstrated that a number of test kits showed considerable quality defects, reducing their applicability to residue control. LODs for urine assayed without a previous clean-up (as recommended by all kit manufacturers) varied between 1.2 and 11.1 ng clenbuterol per ml urine and did thus not meet the requirement of 1 ng/ml of the official residue control plans of the EU Member States.
机译:本文介绍了九种市售的ELISA试剂盒的比较研究结果,该试剂盒用于瘦肉精和其他β2-激动剂,目前正用于欧盟(EU)活体和宰杀动物的兽药残留控制。通过确定测量范围,B / B_0-50%值,测定间和测定内变异和交叉反应以及牛尿中克仑特罗的检测限(LOD)和回收率,可以证明许多检测试剂盒表现出相当大的质量缺陷,降低了其在残留物控制中的适用性。未经事先净化(所有试剂盒制造商建议)的尿液检测限在每毫升尿液中介于1.2克和11.1 ng克仑特罗之间,因此不符合欧盟成员官方残留控制计划中1 ng / ml的要求状态。

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