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首页> 外文期刊>Food additives & contaminants >Natural occurrence of aflatoxins (B-1 and M_1) in feed, plasma and raw milk of lactating dairy cows in Beja, Tunisia, using ELISA
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Natural occurrence of aflatoxins (B-1 and M_1) in feed, plasma and raw milk of lactating dairy cows in Beja, Tunisia, using ELISA

机译：使用ELISA法在突尼斯贝雅泌乳奶牛的饲料，血浆和生乳中自然产生黄曲霉毒素（B-1和M_1）

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摘要

Beja is an agricultural area in northwest Tunisia. It contributes to national needs by offering cereals and milk to the market for human and animal consumption. A small number of studies on mycotoxin occurrence in feedstuffs and raw milk from lactating dairy cows in this region are available. Therefore, 226 samples were collected from farms and local markets during November 2008 until April 2010. Samples consisted of 112 raw cow milk, 56 blood from lactating cows and 58 feed destined for dairy cows. Plasma and feed were analysed for aflatoxin B_1 (AFBi). Milk samples were analysed for aflatoxin M, (AFM_1). All samples were treated using a simultaneous methanolic-aqueous extraction, followed by immunoaffinity column clean-ups and were investigated by competitive enzyme-linked immunoabsorbent assay (ELISA). Recoveries were 80%-95% and 81%-92% for AFB_1 and AFM_1, respectively, while the limit of detection (LOD) was 0.01 ng/kg or μg/1 for both mycotoxins. Results revealed the presence of AFB, in 84.4% of the feed samples (mean 18.7 ± 1.4 μg/kg), and 39.2% of the plasma-examined samples (median 7.1 ± 1.0 μg/l) were found to be contaminated at levels higher than the Tunisian and the European Union (EU) limit for dairy animals, which are 20 and 5 μg/kg in animal feed, respectively. AFM_1 was detected in 60.7% of the cow raw milk samples examined (median 13.6± 1.4 μg/l). Contaminated levels were higher than the EU limit of 0.05 ug/1. It was concluded that more precaution should be taken on hygiene controls in order to prevent fungal contamination.
机译：贝雅是突尼斯西北部的一个农业地区。它通过向人类和动物消费市场提供谷物和牛奶来满足国家需求。关于该地区泌乳奶牛的饲料和生乳中霉菌毒素的少量研究可获。因此，在2008年11月至2010年4月期间，从农场和当地市场收集了226个样品。这些样品包括112头生牛奶，56头泌乳牛的血液和58头奶牛饲料。分析血浆和饲料中的黄曲霉毒素B_1（AFBi）。分析牛奶样品中的黄曲霉毒素M（AFM_1）。所有样品均使用甲醇水溶液同时萃取，然后进行免疫亲和柱净化，并通过竞争性酶联免疫吸附测定（ELISA）进行研究。 AFB_1和AFM_1的回收率分别为80％-95％和81％-92％，而两种霉菌毒素的检出限（LOD）均为0.01 ng / kg或μg/ 1。结果表明，AFB的存在被发现的污染程度较高，其中84.4％的饲料样品（平均18.7±1.4μg/ kg）和39.2％的血浆检查样品（平均7.1±1.0μg/ l）被污染高于突尼斯和欧盟（EU）对奶牛的限制，分别为20和5μg/ kg。在所检查的60.7％的牛奶原料中检测到AFM_1（中值13.6±1.4μg/ l）。污染水平高于欧盟规定的0.05 ug / 1。结论是，应在卫生控制上采取更多预防措施，以防止真菌污染。

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  • 来源
    《Food additives & contaminants》 |2012年第1期|p.11-15|共5页
  • 作者

    Samir Abbes; Jalila Ben Salah-Abbes; Yousra Bouraoui; Sarra Oueslati; Ridha Oueslati;

  • 作者单位

    Unit of Immunology, Environmental Microbiology and Cancerology, Faculty of Sciences Bizerte, University of Carthage, Tunisia,Animal Biotechnology Department, Higher Institute of Biotechnology of Beja, University of Jendouba, Beja, Tunisia;

    Unit of Immunology, Environmental Microbiology and Cancerology, Faculty of Sciences Bizerte, University of Carthage, Tunisia;

    Unit of Immunology, Environmental Microbiology and Cancerology, Faculty of Sciences Bizerte, University of Carthage, Tunisia;

    Unit of Immunology, Environmental Microbiology and Cancerology, Faculty of Sciences Bizerte, University of Carthage, Tunisia;

    Unit of Immunology, Environmental Microbiology and Cancerology, Faculty of Sciences Bizerte, University of Carthage, Tunisia;

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  • 正文语种 eng
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  • 关键词

    raw milk; feedstuff; plasma; aflatoxin B_1; aflatoxin M_1; occurrence; ELISA; risk;

    机译：生乳饲料等离子体;黄曲霉毒素B_1;黄曲霉毒素M_1;发生;ELISA;风险;

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