Isolation, identification and activity of natural antioxidants from horehound (Marrubium vulgare L.) cultivated in Lithuania

Audrius Pukalskas; Petras Rimantas Venskutonis; Sofia Salido; Pieter de Waard; Teris A. van Beek

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Isolation, identification and activity of natural antioxidants from horehound (Marrubium vulgare L.) cultivated in Lithuania

机译：在立陶宛种植的苦薄荷（Marrubium vulgare L.）中天然抗氧化剂的分离，鉴定和活性

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In an earlier screening of Lithuanian plants, horehound (Marrubium vulgare) showed good antioxidant activity and as this species is used in herbal teas and cough pastilles it was selected for further investigation. Some fractions of the aerial parts were strong scavengers of the model free radicals DPPH and ABTS~+. Activity in the β-carotene bleaching assay and the rapeseed oil oxidation assay was lower. Several active compounds were observed in the crude methanol-water extract, and in butanol and methyl tert-butyl ether fractions using HPLC with on-line radical scavenging detection. After multi-step fraction-ation of these fractions five compounds possessing radical scavenging activity were purified and their structures were elucidated by NMR and MS as 5,6-dihydroxy-7,4'-dimethoxyflavone (syn. ladanein), 7-O-β-glucopyranosyl luteolin, 7-O-β-glucuronyl luteolin, verbascoside and forsythoside B. Their activities were tested in off-line DPPH and ABTS+' free radical scavenging assays, and compared with the antioxidants rosmarinic acid and Trolox.

机译：在较早的立陶宛植物筛选中，苦薄荷（Marrubium vulgare）具有良好的抗氧化活性，由于该物种被用于凉茶和咳嗽锭剂中，因此被选作进一步研究。空气部分的某些部分是模型自由基DPPH和ABTS〜+的强清除剂。 β-胡萝卜素漂白法和菜籽油氧化法的活性较低。使用具有在线自由基清除检测功能的HPLC，在粗甲醇-水萃取液以及丁醇和甲基叔丁基醚馏分中观察到了几种活性化合物。在对这些馏分进行多步分馏后，纯化了具有自由基清除活性的五种化合物，并通过NMR和MS阐明了其结构，分别为5,6-二羟基-7,4'-二甲氧基黄酮（合成拉丹宁），7-O- β-吡喃葡萄糖基木犀草素，7-O-β-葡萄糖醛酸木犀草素，马齿side甙和连翘甙B.它们的活性在离线DPPH和ABTS +自由基清除试验中进行了测试，并与抗氧化剂迷迭香酸和Trolox进行了比较。

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来源
《Food Chemistry》 |2012年第3期|p.695-701|共7页
作者
Audrius Pukalskas; Petras Rimantas Venskutonis; Sofia Salido; Pieter de Waard; Teris A. van Beek;
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作者单位

Department of Food Technology, Kaunas University of Technology, Radvilenu pl. 19,17-50254 Kaunas, Lithuania;

Department of Food Technology, Kaunas University of Technology, Radvilenu pl. 19,17-50254 Kaunas, Lithuania;

Dpto. Quimica Inorganica y Organka, Facultad de Ciencias Experimentates, Universidad dejaen, 23071 Jaen, Spain;

Wageningen NMR Centre, Wageningen University, Dreijenlaan 3, 6703 HA Wageningen, The Netherlands;

Laboratory of Organic Chemistry, Natural Products Chemistry and Microfluidics Group, Wageningen University, Dreijenplein 8, 6703 HB Wageningen, The Netherlands;

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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);
原文格式 PDF
正文语种 eng
中图分类
关键词
horehound; marrubium vulgare; antioxidants; flavonoids;

机译：恐怖猎犬芸苔;抗氧化剂;类黄酮;

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Isolation, identification and activity of natural antioxidants from horehound (Marrubium vulgare L.) cultivated in Lithuania

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