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首页> 外文期刊>Food Chemistry >Effect of reactant concentrations on the Maillard reaction in a fructose-glycine model system and the inhibition of black tiger shrimp polyphenoloxidase
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Effect of reactant concentrations on the Maillard reaction in a fructose-glycine model system and the inhibition of black tiger shrimp polyphenoloxidase

机译：反应物浓度对果糖-甘氨酸模型系统中美拉德反应的影响以及对黑虎虾多酚氧化酶的抑制作用

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摘要

The characteristics and the inhibitory activity towards black tiger shrimp polyphenoloxidase of Maillard reaction products (MRPs), prepared by heating an equimolar mixture of fructose and glycine at various concentrations (0.75-30 mM) at 100℃ for 12 h, were investigated. Increase in the intermediate products was observed with increasing reactant concentration, as evidenced by the increase in A_(294) and fluorescence intensity. Furthermore, development of browning (A_(420)) was noticeable when the reactant concentration increased. The inhibitory activity of MRPs towards PPO gradually increased when the concentration of each reactant increased and reached a maximum at 30 mM (80% inhibition). The increase in the inhibitory activity of MRPs was coincidental with the increase in the reducing power, A_(294), fluorescence intensity, as well as browning intensity. Generally, the development of Maillard reaction products was associated with decrease in pH and loss of reducing sugar and free amino groups, with coincidental increase in reducing power and copperchelating property. MRPs with the reactant concentration of 4.5-30 mM were able to chelate the copper ion. Therefore, the inhibitory activity of MRPs towards browning, induced by PPO, was most likely due to their copper-chelating property as well as reducing power.
机译：研究了将等浓度的果糖和甘氨酸等摩尔混合物在0.75-30 mM下于100℃加热12小时制得的美拉德反应产物（MRPs）的特性和对黑虎虾多酚氧化酶的抑制活性。随着反应物浓度的增加，观察到中间产物的增加，这由A_（294）和荧光强度的增加证明。此外，当反应物浓度增加时，褐变明显（A_（420））。当每种反应物的浓度增加时，MRP对PPO的抑制活性逐渐增加，并在30 mM时达到最大值（抑制80％）。 MRPs抑制活性的增加与还原能力，A_（294），荧光强度以及褐变强度的增加是一致的。通常，美拉德反应产物的发展与pH的降低以及还原糖和游离氨基的损失有关，同时与还原能力和铜螯合性能的增加有关。反应物浓度为4.5-30 mM的MRP能够螯合铜离子。因此，由PPO诱导的MRP对褐变的抑制活性最可能是由于其铜螯合性能以及降低的功率。

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