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首页> 外文期刊>Food Chemistry >Studies on submerged fermentation of Pleurotus tuber-regium (Fr.) Singer―Part 1: physical and chemical factors affecting the rate of mycelial growth and bioconversion efficiency
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Studies on submerged fermentation of Pleurotus tuber-regium (Fr.) Singer―Part 1: physical and chemical factors affecting the rate of mycelial growth and bioconversion efficiency

机译：杏鲍菇深层发酵研究第1部分：影响菌丝生长速度和生物转化效率的理化因素

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摘要

Submerged fermentation of the mycelium of Pleurotus tuber-regium was investigated by studying the physical parameters including the amount of inoculum and duration of fermentation as well as different chemical factors including the carbon and nitrogen sources. The fermentation conditions was set at an orbital shaking of 200 +- 10 rpm at 30 +- 1℃ with a basal medium containing 30 g fructose, 2 g peptone, 2 g yeast extract, 1 g KH_2PO_4, 0.6 g MgSO_4.7H_2O per 11 of distilled water. The amount of mycelial inoculum was set at 10 ml/l of medium. Growth of mycelium using glucose and fructose had a relatively shorter lag phase than that of maize starch with maximum growth on different periods of fermentation. Nitrogen utilization of yeast extract was better than that of peptone when monosaccharide was used as the sole carbon source. Maximum bioconversion efficiency found was about 40%, which was much higher than those reported previously for P. tuber-regium. In the scale-up fermentation, basal medium with fructose gave higher mycelial yield than that of glucose.
机译：通过研究包括接种量和发酵持续时间在内的物理参数以及包括碳源和氮源在内的不同化学因素，研究了白灵菇菌丝体的深层发酵。发酵条件设定为在30±1℃的条件下以200±10 rpm的轨道振动，其中每10公斤的基础培养基含30 g果糖，2 g蛋白ept，2 g酵母提取物，1 g KH_2PO_4、0.6 g MgSO_4.7H_2O蒸馏水。菌丝体接种量设置为10 ml / l的培养基。与玉米淀粉相比，使用葡萄糖和果糖的菌丝体的生长具有相对较短的滞后期，并且在不同的发酵时期具有最大的生长。当单糖用作唯一碳源时，酵母提取物的氮利用效率优于蛋白ept。发现的最大生物转化效率约为40％，这比以前报道的块茎疫病菌的效率高得多。在扩大规模的发酵中，含果糖的基础培养基的菌丝体产量高于葡萄糖。

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