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首页> 外文期刊>Lebensmittelchemie >A Rapid Method for Determination of Vitamin E and Radical Scavenging Activity in Plasma and Tissues
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A Rapid Method for Determination of Vitamin E and Radical Scavenging Activity in Plasma and Tissues

机译:一种测定血浆和组织中维生素E和自由基清除活性的快速方法

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摘要

Vitamin E is of particular importance in nutrition through preventing membranes from free radical oxidative damage. An iso-cratic normal phase high-performance liquid chromatography (NP-HPLC) method for vitamin E analysis using a Lichrosorb Si 60 silica column linked with an ultraviolet (UV) detector was developed. Isoocta-ne/ethylacetate (96:4 v/v) was used as the eluant for the simultaneous elution of vitamin E. The vitamin E was extracted from tissues and plasma of two groups of twelve's Long-Evans rats which fed different levels of DHA and GLA [group 1; a) 1% DHA, b) 2% DHA and c) 3%DHA; group 2; ⅰ) 1% GLA, ⅱ) 3% GLA and ⅲ) 3%GLA]. Each group was fed diets containing 10% by weight of lipid for a period of 8 weeks. The diets contain the same ratio of n-6: n-3 (5:1).
机译:维生素E通过防止膜受到自由基氧化损伤,在营养中特别重要。开发了一种等度正相高效液相色谱(NP-HPLC)方法,该方法使用与紫外(UV)检测器连接的Lichrosorb Si 60硅胶柱进行维生素E分析。异辛烷/乙酸乙酯(96:4 v / v)用作同时洗脱维生素E的洗脱液。维生素E从两组十二只Long-Evans大鼠的组织和血浆中提取,分别喂养了不同水平的DHA和GLA [第1组; a)1%DHA,b)2%DHA和c)3%DHA; 2组ⅰ)1%GLA,ⅱ)3%GLA和ⅲ)3%GLA]。每组喂食含10%(重量)脂质的饮食,持续8周。饮食中n-6:n-3(5:1)的比例相同。

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