Changes in the stability and antioxidant activities of different molecular weight bioactive peptide extracts obtained from beef during in vitro human digestion by gut microbiota

Lee Seung Yun; Lee Da Young; Hur Sun Jin

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Changes in the stability and antioxidant activities of different molecular weight bioactive peptide extracts obtained from beef during in vitro human digestion by gut microbiota

机译：通过肠道微生物在体外人体消化期间从牛肉中获得的不同分子量生物活性肽提取物的稳定性和抗氧化活性的变化

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This study was conducted to determine changes in the stability and antioxidant activity of extracts of bioactive peptides with different molecular weights (3 and 10 kDa) obtained from beef myofibrillar protein using commercial enzymes (alkaline-AK and papain) during in vitro human digestion by gut microbiota. After the digestion in the large intestine, the stability of the bioactive peptide extracts decreased regardless of their molecular weight. However, the peptides obtained following alkaline-AK treatment were less stable than those obtained following papain digestion. The radical scavenging activities of the peptide extracts also decreased during in vitro human digestion, regardless of the molecular weights of the peptides and the commercial enzymes used. These results indicate that the stability and antioxidative activity of the bioactive peptides were affected by the digestion process by the gut microbiota. This study provides data supporting the changes in the stability and bioavailability of functional materials within the human body.

机译：进行该研究以确定在体外人体消化期间使用商业酶（碱-αk和毒素）在牛肉肌原纤维蛋白中获得的不同分子量（<3和<10kDa）的生物活性肽提取物的稳定性和抗氧化活性的变化通过肠道微生物。在大肠中消化后，无论其分子量如何，生物活性肽提取物的稳定性降低。然而，在碱性-AK处理后获得的肽比蛋白酶消化所得的肽稳定。无论肽的分子量和使用的商业酶如何，肽提取物的自由基清除活性也降低。这些结果表明，生物活性肽的稳定性和抗氧化活性受到肠道微生物的消化过程的影响。本研究提供了支持人体内功能材料的稳定性和生物利用度的变化的数据。

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来源
《Food research international》 |2021年第3期|110116.1-110116.7|共7页
作者
Lee Seung Yun; Lee Da Young; Hur Sun Jin;
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作者单位

Chung Ang Univ Dept Anim Sci & Technol 4726 Seodong Daero Anseong 456756 Gyeonggi Do South Korea;

Chung Ang Univ Dept Anim Sci & Technol 4726 Seodong Daero Anseong 456756 Gyeonggi Do South Korea;

Chung Ang Univ Dept Anim Sci & Technol 4726 Seodong Daero Anseong 456756 Gyeonggi Do South Korea;

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收录信息美国《科学引文索引》(SCI);美国《工程索引》(EI);
原文格式 PDF
正文语种 eng
中图分类
关键词
Stability; Bioactive peptide; In vitro digestion; Gut microbiota;

机译：稳定性;生物活性肽;体外消化;肠道微生物;

Changes in the stability and antioxidant activities of different molecular weight bioactive peptide extracts obtained from beef during in vitro human digestion by gut microbiota

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