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首页> 外文期刊>Food science and technology research >Extraction Optimization, Preliminary Characterization and Antioxidant Activity of Glycoproteins from the Muscle of Sepia pharaonis
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Extraction Optimization, Preliminary Characterization and Antioxidant Activity of Glycoproteins from the Muscle of Sepia pharaonis

机译：法老乌贼肌肉中糖蛋白的提取优化，初步表征和抗氧化活性

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摘要

The extraction, purification, preliminary characterization and antioxidant activity of glycoproteins from Sepia pharaonis muscle (SPMG) were investigated. The optimal extraction conditions were obtained using a Box-Behnken design. The crude SPMG was purified by DEAF-Cellulose 52 ion-exchange and Sephadex G-100 gel chromatography and two fractions (SPMG-I and SPMG-II) were obtained. SPMG-I and SPMG-II were rich in Glu, Asp, Leu, Arg and Lys with the molecular weight of 42.5 and 36.3 kDa, respectively. The glucose was the predominant monosaccharide composition of SPMG-I and SPMG-II. The existence of O-glycosidic linkage in SPMG-I and SPMG-II was demonstrated by a beta-elimination reaction. SPMG-I and SPMG-II were characterized by FT-IR. SPMG, SPMG-I and SPMG-II showed excellent antioxidant activities and SPMG-I exhibited the highest antioxidant ability. These may provide theoretical basis for further system research and rational development and utilization of SPMG resource.
机译：研究了法老棕褐色肌肉（SPMG）糖蛋白的提取，纯化，初步表征和抗氧化活性。最佳提取条件是使用Box-Behnken设计获得的。通过DEAF-纤维素52离子交换和Sephadex G-100凝胶色谱法纯化粗SPMG，得到两个级分（SPMG-I和SPMG-II）。 SPMG-I和SPMG-II富含Glu，Asp，Leu，Arg和Lys，分子量分别为42.5和36.3 kDa。葡萄糖是SPMG-I和SPMG-II的主要单糖组成。通过β-消除反应证明了SPMG-I和SPMG-II中O-糖苷键的存在。 SPMG-I和SPMG-II用FT-IR表征。 SPMG，SPMG-I和SPMG-II具有出色的抗氧化活性，而SPMG-I具有最高的抗氧化能力。这些可以为进一步的系统研究和SPMG资源的合理开发利用提供理论依据。

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  • 来源
    《Food science and technology research》 |2016年第1期|39-52|共14页
  • 作者

    Dai Hongjie; Sun Yulin; Zheng Xiaolin; Feng Zixin; Wen Jing; Zhao Juan; Chen Daohai;

  • 作者单位

    Lingnan Normal Univ, Round Beibu Gulf Inst Protect & Utilizat Marine A, Zhanjiang 524048, Peoples R China|Lingnan Normal Univ, Life Sci & Technol Sch, Zhanjiang 524048, Peoples R China|Zhejiang Gongshang Univ, Coll Food & Biol Engn, Hangzhou 310018, Zhejiang, Peoples R China;

    Lingnan Normal Univ, Round Beibu Gulf Inst Protect & Utilizat Marine A, Zhanjiang 524048, Peoples R China|Lingnan Normal Univ, Life Sci & Technol Sch, Zhanjiang 524048, Peoples R China;

    Zhejiang Gongshang Univ, Coll Food & Biol Engn, Hangzhou 310018, Zhejiang, Peoples R China;

    Lingnan Normal Univ, Life Sci & Technol Sch, Zhanjiang 524048, Peoples R China;

    Lingnan Normal Univ, Round Beibu Gulf Inst Protect & Utilizat Marine A, Zhanjiang 524048, Peoples R China|Lingnan Normal Univ, Life Sci & Technol Sch, Zhanjiang 524048, Peoples R China;

    Lingnan Normal Univ, Round Beibu Gulf Inst Protect & Utilizat Marine A, Zhanjiang 524048, Peoples R China|Lingnan Normal Univ, Life Sci & Technol Sch, Zhanjiang 524048, Peoples R China;

    Lingnan Normal Univ, Round Beibu Gulf Inst Protect & Utilizat Marine A, Zhanjiang 524048, Peoples R China;

  • 收录信息 美国《科学引文索引》(SCI);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Sepia pharaonis; glycoproteins; extraction; characterization; antioxidant activity;

    机译：法老棕褐色;糖蛋白;提取;表征;抗氧化活性;

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