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Analysis of Novel Soybean Sprout Allergens That Cause Food-Induced Anaphylaxis

机译：引起食物过敏的新型大豆新芽变态反应原的分析

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摘要

Several soybean- and soy product-based allergens have been reported. However, a full analysis of soybean sprout allergens remains to be conducted. Soybean sprout allergy is related to soy intolerance. Here we report a case of anaphylaxis induced by soybean sprouts in a 49-year-old woman. We performed a skin prick test, allergen-specific IgE identification using microarray assay (ImmunoCAP®-ISAC), SDS-PAGE, and immunoblotting for allergen analysis. The ImmunoCAP®-ISAC microarray assay detected Gly m 4 in the soybean sprout. However, immunoblotting revealed two 100-kDa protein bands that did not correspond to Gly m 4 (17 kDa). Therefore, we propose that this patient was not only sensitized to the Gly m 4 allergen, but also to the novel 100-kDa protein.
机译：已经报道了几种基于大豆和大豆产品的过敏原。但是，大豆芽变应原的全面分析仍有待进行。大豆新芽过敏与大豆不耐症有关。在这里，我们报道了一名49岁妇女因豆芽引起的过敏反应。我们进行了皮肤点刺测试，使用微阵列测定（ImmunoCAP®-ISAC）进行过敏原特异性IgE鉴定，SDS-PAGE和免疫印迹进行过敏原分析。 ImmunoCAP®-ISAC芯片检测可检测出大豆芽中的Gly m 4。然而，免疫印迹揭示了两个不对应于Gly m 4（17 kDa）的100 kDa蛋白带。因此，我们建议该患者不仅对Gly m 4过敏原敏感，而且对新型100 kDa蛋白敏感。

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  • 来源
    《Food science and technology research》 |2013年第4期|617-621|共5页
  • 作者

    Ryoko Nakagawara; Yasuharu Itagaki; Masumi Kohno; Setsuko Matsukura; Maki Miyazawa; Kenichi Kumasaka; Takashi Kojima; Zenro Ikezawa; Michiko Aihara;

  • 作者单位

    Department of Environmental Immuno-Dermatology, Yokohama City University, Graduate School of Medicine, Kanagawa, Japan ,Yokohama City University Medical Center, Kanagawa, Japan;

    Department of Environmental Immuno-Dermatology, Yokohama City University, Graduate School of Medicine, Kanagawa, Japan ,Department of Health and Nutrition, Hokkaido Bunkyo University, Hokkaido, Japan ,Kanagawa Academy of Science and Technology, Kanagawa, Japan;

    Yokohama City University Medical Center, Kanagawa, Japan;

    Yokohama City University Medical Center, Kanagawa, Japan;

    Kanagawa Prefectural Institute of Public Health, Kanagawa, Japan;

    Kanagawa Prefectural Institute of Public Health, Kanagawa, Japan;

    Kanagawa Prefectural Institute of Public Health, Kanagawa, Japan;

    Department of Environmental Immuno-Dermatology, Yokohama City University, Graduate School of Medicine, Kanagawa, Japan;

    Department of Environmental Immuno-Dermatology, Yokohama City University, Graduate School of Medicine, Kanagawa, Japan;

  • 收录信息 美国《科学引文索引》(SCI);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    food allergy; soybean sprout; allergen analysis; anaphylaxis;

    机译：食物过敏;大豆芽过敏原分析过敏反应;

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