首页> 外文期刊>Food science and technology research >Isolation of Lactic Acid Bacteria from Takanazuke as a Starter Strain to Reduce Added Salt and Stabilize Fermentation
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Isolation of Lactic Acid Bacteria from Takanazuke as a Starter Strain to Reduce Added Salt and Stabilize Fermentation

机译:从Takanazuke分离乳酸菌作为发酵菌株以减少添加的盐并稳定发酵

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摘要

Lactic acid bacteria (LAB) were isolated from takanazuke produced by using harvested takana in the Aso area. Eight strains were identified from the results of partial 16S rRNA gene sequence analysis: Enterococcus faecium, Leuconostoc mesenteroides, Lactobacillus sakei, L. curvatus, L. (para)plantarum, L. brevis, L. parabrevis, and Pediococcus parvulus. Leu. mesenteroides shared the same sequence with L. delbrueckii subsp. bulgaricus. However, they were differentiated by microscopic observation and a sugar assimilation test. In the fermentation tests using MRS and takana juice medium, L. sakei showed a different ratio of L-lactate to D-lactate from L. curvatus, although they had a similar 16S rRNA gene sequence. This difference was supposed to be based on the inhibitory effect of sodium acetate on lactate racemase. L. (para)plantarum and P. parvulus were selected as starter LABs since they decreased pH and produced high concentrations of lactate in both MRS and tanaka juice media.
机译:乳酸菌(LAB)是从在阿苏地区使用收获的高ana生产的高铁中分离出来的。从部分16S rRNA基因序列分析的结果中鉴定出八个菌株:粪肠球菌,肠膜明串珠菌,清酒乳杆菌,弯豆乳杆菌,植物乳杆菌,短乳杆菌,副乳杆菌和小球藻。亮mesenteroides与L. delbrueckii亚种共有相同的序列。保加利亚然而,它们通过显微镜观察和糖同化试验来区分。在使用MRS和takana汁液培养基进行的发酵测试中,日本酒L.乳酸菌与弯曲乳杆菌的L-乳酸与D-乳酸的比例不同,尽管它们具有相似的16S rRNA基因序列。该差异被认为是基于乙酸钠对乳酸消旋酶的抑制作用。之所以选择植物乳杆菌和小生乳酸杆菌作为起始乳酸菌,是因为它们降低了pH值,并且在MRS和田中果汁中均产生了高浓度的乳酸。

著录项

  • 来源
    《Food science and technology research》 |2013年第4期|577-582|共6页
  • 作者单位

    Graduate School of Science and Technology, Kumamoto University, 2-39-1 Kurokami Chuou-ku, Kumamoto City, Kumamoto 860-8555,Japan;

    Graduate School of Science and Technology, Kumamoto University, 2-39-1 Kurokami Chuou-ku, Kumamoto City, Kumamoto 860-8555,Japan;

    Graduate School of Science and Technology, Kumamoto University, 2-39-1 Kurokami Chuou-ku, Kumamoto City, Kumamoto 860-8555,Japan;

    Graduate School of Science and Technology, Kumamoto University, 2-39-1 Kurokami Chuou-ku, Kumamoto City, Kumamoto 860-8555,Japan;

    Graduate School of Science and Technology, Kumamoto University, 2-39-1 Kurokami Chuou-ku, Kumamoto City, Kumamoto 860-8555,Japan;

  • 收录信息 美国《科学引文索引》(SCI);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    takanazuke; lactic acid bacteria; isolation; lactate racemase; acetate inhibition;

    机译:高kan乳酸菌隔离;乳酸消旋酶;醋酸盐抑制;

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