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首页> 外文期刊>Forestry: An International Journal of Forest Research >Testing novel cytokinins for improved in vitro adventitious shoots formation and subsequent ex vitro performance in Pinus radiata
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Testing novel cytokinins for improved in vitro adventitious shoots formation and subsequent ex vitro performance in Pinus radiata

机译：测试新型细胞分裂素可改善辐射松的体外不定芽形成和随后的离体表现

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摘要

The aim of this study was to assess the effect of cytokinins not commonly used for shoot induction from zygotic embryos of Pinus radiata D. Don. The influence of in vitro shoot and root induction treatments on the subsequent ex vitro development of the regenerated plants was also tested. Embryos were cultured with benzyladenine (BA), thidiazuron (T) and two cytokinins not previously assayed in radiata pine organogenesis (meta-topolin (m-T) and zeatin (Z)) in a range of concentrations and induction periods. Shoot induction treatments were assayed in seeds from different geographical origins to obtain wider conclusions. We analysed the effect of these cytokinin treatments on in vitro rooting with different auxins ((indole-3-butyric acid (IBA) and 1-naphtalene acetic acid (NAA)) and the traditionally used mixture. After in vitro rooting, the plantlets were acclimatized and their ex vitro behaviour was evaluated. Shoot induction treatments with 1 μM BA for 2 weeks, 4.4 μM BA for 3 weeks or 1 μM Z for 3 weeks were more effective than the other treatments. An interaction between in vitro shoot and root induction treatments was observed. IBA was more efficient for plant production because the explants rooted in this auxin had better survival rates in the greenhouse.
机译：这项研究的目的是评估不常用于辐射松（Pinus radiata D. Don）合子胚芽诱导的细胞分裂素的作用。还测试了体外芽和根诱导处理对再生植物随后离体发育的影响。用苄基腺嘌呤（BA），噻二唑隆（T）和两种以前未在辐射松器官发生中测定的细胞分裂素（间位拓扑素（m-T）和玉米素（Z））培养一系列浓度和诱导期的胚。对来自不同地理来源的种子进行了芽诱导处理，以获得更广泛的结论。我们分析了这些细胞分裂素处理对不同生长素（（吲哚-3-丁酸（IBA）和1-萘乙酸（NAA））和传统使用的混合物在体外生根的影响。适应环境并评估其离体行为。用1μMBA 2周，4.4μMBA 3周或1μMZ 3周的诱导诱导处理比其他处理更有效。由于生长在这种生长素中的外植体在温室中具有更好的存活率，因此IBA对植物的生产更为有效。

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