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首页> 外文期刊>Fresenius Environmental Bulletin >BIODEGRADATION OF 2,4-DICHLOROPHENOXYACETIC ACID (2,4-D) HERBICIDE BY PSEUDOMONAS SPP. STRAINS
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BIODEGRADATION OF 2,4-DICHLOROPHENOXYACETIC ACID (2,4-D) HERBICIDE BY PSEUDOMONAS SPP. STRAINS

机译：假单胞菌SPP对2,4-二氯苯氧乙酸（2,4-D）的除草剂生物降解。应变

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摘要

In this study, the ability of 2,4-dichlorophenoxyacetic acid (2,4-D) tolerance and degradation of Pseudomonas spp. isolates was investigated. 20 strains of Pseudomonas spp. were tested for their tolerance to 2,4-D. All the strains exhibited variation in sensitivity at 50-1000 ppm 2,4-D concentration but none of the strains were completely inhibited. When compared with the control tubes, all strains exhibited intensive growth but 3 strains (B7, B8, B12), are less intensive than others. P. aeruginosa B1 strain was the one with most resistant to 2,4-D concentrations tested. On the contrary, P. fluorescens B7 strain was more sensitive to 2,4-D at these concentrations. Growth of the 20 strains examined remained unaffected at concentrations between 50 and 200 ppm whereas some of the strains showed sensitivity at 850-1000 ppm concentrations. The 20 strains of Pseudomonas spp. were further screened for 2,4-D degrading capacity. Only one strain (Bl) having appreciable 2,4-D herbicide degrading capability was selected. Tolerance to and degradation of 2,4-D by B1 was significantly greater than others. The degradation capacity of 2,4-D herbicide of this culture were performed by HPLC. HPLC data analysis has revealed 24, 31, 32, 33% degradation of 2,4-D respectively in 24, 48, 72 and 96 h of incubation at 37℃ . HPLC and spectrophotometric measures of the degradation capacity of Bl strain showed that it had the ability to degrade 2,4-D herbicide. This study suggests that resistance to 2,4-D and ability to degradation were achieved through the cells of Bl. This strain could be considered as highly beneficial for application of bioremediation of 2,4-D herbicide pollutants.
机译：在这项研究中，2，4-二氯苯氧基乙酸（2,4-D）的耐受性和假单胞菌的降解能力。分离株进行了调查。 20株假单胞菌属。被测试对2，4-D的耐受性。在50-1000ppm的2，4-D浓度下，所有菌株均表现出敏感性变化，但是没有一个菌株被完全抑制。当与对照管比较时，所有菌株均表现出密集的生长，但是3个菌株（B7，B8，B12）的强度低于其他菌株。铜绿假单胞菌B1菌株是对2,4-D浓度最有抵抗力的菌株。相反，在这些浓度下，荧光假单胞菌B7菌株对2，4-D更敏感。在浓度为50至200 ppm的情况下，所检查的20个菌株的生长均未受影响，而其中某些菌株在850-1000 ppm的浓度下显示出敏感性。假单胞菌的20株。进一步筛选了2,4-D降解能力。仅选择了具有明显的2，4-D除草剂降解能力的菌株（B1）。 B1对2,4-D的耐受性和降解能力明显高于其他人。该培养物的2，4-D除草剂的降解能力通过HPLC进行。 HPLC数据分析表明，在37℃下孵育24、48、72和96小时，分别降解了2,4-D 24、31、32、33％。 HPLC和分光光度法测定B1菌株的降解能力表明其具有降解2，4-D除草剂的能力。该研究表明通过B1细胞获得了对2，4-D的抗性和降解能力。该菌株被认为对2,4-D除草剂污染物的生物修复应用非常有利。

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