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Optimization of media components and culture conditions for polyhydroxyalkanoates production by Bacillus megaterium

机译：芽孢杆菌生产培养基组分及培养条件的优化芽孢杆菌生产

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摘要

Polyhydroxybutyrate (PHB) accumulating Bacillus megaterium was isolated from marine water. To increase the PHB productivity by Bacillus megaterium, steps were taken to evaluate the effects of carbon sources (arabinose, glucose, glycerol, lactose, lactic acid, mannitol, sodium acetate, starch and sucrose at a level of 20 g/L), nitrogen sources (ammonium chloride, ammonium sulphate, glycine, potassium nitrate, protease peptone and urea at a level of 2 g/L) and different pH. A maximum yield of 2.74 g/L of PHB was achieved for glucose as the carbon source and ammonium sulphate as the nitrogen source at pH 7. The optimized conditions were further used for batch fermentation throughout 72 h. Significantly maximum PHB of 5.61 g/L was obtained in a laboratory scale bioreactor at 64 h. The extracted polymer was compared with the authentic PHB and was confirmed to be PHB using FTIR, H-1 NMR, DSC and TGA analysis, respectively.
机译：从海水中分离出积聚芽孢杆菌的多羟基丁酸酯（PHB）。为了增加芽孢杆菌生产率的PHB生产力，采取步骤来评估碳源的影响（阿拉伯糖，葡萄糖，甘油，乳糖，乳酸，甘露醇，乙酸钠，淀粉和蔗糖在20g / L），氮气水平来源（氯化铵，硫酸铵，甘氨酸，硝酸钾，蛋白酶蛋白胨和尿素，含量为2g / L）和不同的pH。为葡萄糖作为碳源和硫酸铵作为氮源作为氮源的最大收率达到2.74g / L的PHB。优化条件进一步用于整个72小时的分批发酵。在64小时的实验室测量生物反应器中获得了5.61g / L的显着最大pHB。将提取的聚合物与真实的PHB进行比较，并使用FTIR，H-1 NMR，DSC和TGA分析证实是PHB。

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  • 来源
    《Fuel》 |2020年第jul1期|117522.1-117522.9|共9页
  • 作者

    Mohanrasu K.; Rao R. Guru Raj; Dinesh G. H.; Zhang Kunyu; Prakash G. Siva; Song Dong-Po; Muniyasamy Sudhakar; Pugazhendhi Arivalagan; Jeyakanthan J.; Arun A.;

  • 作者单位

    Alagappa Univ Dept Energy Sci Karaikkudi Tamil Nadu India|Alagappa Univ Dept Microbiol Karaikkudi Tamil Nadu India;

    Alagappa Univ Dept Bioinformat Karaikkudi Tamil Nadu India;

    Alagappa Univ Dept Energy Sci Karaikkudi Tamil Nadu India|Alagappa Univ Dept Microbiol Karaikkudi Tamil Nadu India;

    Tianjin Univ Sch Chem Engn & Technol Tianjin Peoples R China;

    Alagappa Univ Dept Energy Sci Karaikkudi Tamil Nadu India|Alagappa Univ Dept Microbiol Karaikkudi Tamil Nadu India;

    Tianjin Univ Sch Mat Sci & Engn Tianjin Peoples R China;

    CSIR Mat Sci & Mfg Polymers & Composites Competence Area POB 1124 ZA-6000 Port Elizabeth South Africa|Nelson Mandela Metropolitan Univ Fac Sci Dept Chem POB 77000 ZA-6031 Port Elizabeth South Africa;

    Ton Duc Thang Univ Fac Environm & Labour Safety Innovat Green Prod Synth & Renewable Environm Dev Ho Chi Minh City Vietnam;

    Alagappa Univ Dept Bioinformat Karaikkudi Tamil Nadu India;

    Alagappa Univ Dept Microbiol Karaikkudi Tamil Nadu India;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Polyhydroxybutyrates (PHAs); Bioreactor; Thermo gravimetric analysis (TGA); Bacillus megaterium; NMR;

    机译：聚羟基丁酸酯（相）;生物反应器;热重分析（TGA）;芽孢杆菌兆;芽孢杆菌;

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