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Developing fourth-generation biofuels secreting microbial cell factories for enhanced productivity and efficient product recovery; a review

机译：开发第四代生物燃料，分泌微生物细胞厂，以提高生产力和高效产品回收; 回顾

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摘要

Fourth generation (4G) biofuels have been found compatible with engines, storage systems, and transport facilities. Due to advances in synthetic biology and genetic engineering tools, considerable progress has been made in producing 4G biofuels including high-carbon alcohols, long-chain hydrocarbons, terpenoids-based, and/or fatty-acid derived biofuels using Escherichia coli, Saccharomyces cerevisiae, Pichia pastoris, Yarrowia lypolytica, and Zymomonas mobilis as microbial cellular factories. However, these microbial platforms face various challenges including low product titers, low product yields, difficult product recovery incurring intensive energy consumption, and discharge of large amounts of wastewater. All these issues make the production and recovery of these biofuels expensive and commercially non-competitive. However, various genetic manipulation approaches including overexpression of heat-shock proteins, enhanced production of precursor molecules, regulating redoxbalance, and membrane engineering have been employed to cope with these challenges. This review discusses the progress made in the molecular approaches for the enhanced biosynthesis and easier recovery of these biofuels through employing the secreting microbial cell factories. Besides, based on the data published on membrane transporters in previous fifteen years, selected fungal and bacterial membrane efflux pumps are studied to evaluate their biofuel secretion potential as future targets to be employed in the biofuel secreting microbial cell factories to achieve commercial robustness in the future.
机译：第四代（4G）生物燃料已与发动机，储存系统和运输设施兼容。由于合成生物学和遗传工程工具的进步，在生产4g生物燃料中，包括高碳醇，长链烃，萜类化合物和/或脂肪酸衍生的生物燃料，使用大肠杆菌，酿酒酵母酿酒蛋白， Pichia Pastoris，Yarrowia Lypolytica和Zymomonas Mobilis作为微生物细胞工厂。然而，这些微生物平台面临各种挑战，包括低产物滴度，低产物产量，难度的产物回收，强化能耗，以及大量废水的排出。所有这些问题都使这些生物燃料的生产和恢复昂贵且商业地不竞争。然而，采用各种遗传操纵方法，包括过表达的热休克蛋白，增强的前体分子生产，调节雷氧基抑制和膜工程，以应对这些挑战。本综述讨论了通过使用分泌微生物细胞工厂来增强生物合成的分子方法的进展，并更容易地恢复这些生物燃料。此外，基于前五十五年的膜转运蛋白上发表的数据，研究了所列的真菌和细菌膜的流出泵，以评估他们在生物燃料中的未来目标中使用的生物燃料分泌潜力，以实现未来的商业鲁棒性。 。

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  • 来源
    《Fuel》 |2021年第15期|120858.1-120858.9|共9页
  • 作者

    Malik Sana; Shahid Ayesha; Liu Chen-Guang; Khan Aqib Zafar; Nawaz Muhammad Zohaib; Zhu Hui; Mehmood Muhammad Aamer;

  • 作者单位

    Govt Coll Univ Faisalabad Dept Bioinformat & Biotechnol Bioenergy Res Ctr Faisalabad Pakistan;

    Govt Coll Univ Faisalabad Dept Bioinformat & Biotechnol Bioenergy Res Ctr Faisalabad Pakistan;

    Shanghai Jiao Tong Univ Sch Life Sci & Biotechnol Joint Int Res Lab Metab & Dev Sci State Key Lab Microbial Metab Shanghai Peoples R China;

    Shanghai Jiao Tong Univ Sch Life Sci & Biotechnol Joint Int Res Lab Metab & Dev Sci State Key Lab Microbial Metab Shanghai Peoples R China;

    Xiamen Univ Coll Ocean & Earth Sci Inst Marine Microbes & Ecospheres State Key Lab Marine Environm Sci Xiamen Peoples R China;

    Sichuan Univ Sci & Engn Sch Bioengn Zigong Peoples R China;

    Sichuan Univ Sci & Engn Sch Bioengn Zigong Peoples R China|Govt Coll Univ Faisalabad Dept Bioinformat & Biotechnol Bioenergy Res Ctr Faisalabad Pakistan;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Advanced biofuels; Heterologous synthesis; Membrane efflux pumps; Product yields and titers; Cytotoxicity;

    机译：先进的生物燃料;异源合成;膜流动泵;产品产量和滴度;细胞毒性;

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