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首页> 外文期刊>Fuel >Evaluation of the deteriogenic microbial community using qPCR, n-alkanes and FAMEs biodegradation in diesel, biodiesel and blends (B5, B10, and B50) during storage
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Evaluation of the deteriogenic microbial community using qPCR, n-alkanes and FAMEs biodegradation in diesel, biodiesel and blends (B5, B10, and B50) during storage

机译：使用qPCR，正构烷烃和FAME在存储期间在柴油，生物柴油和混合物（B5，B10和B50）中的生物降解评估微生物

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摘要

Understanding the changes in microbial community structure and its impact during the storage of diesel and biodiesel is critical to prevent damage to the system and to ensure a good quality product to consumers. In this study, the storage of pure diesel (B0), diesel and biodiesel blends (B5, B10, and B50), and pure biodiesel (B100) was simulated. During the simulation, and after contamination with inoculum, microbial growth was monitored through biomass formation. Fungi and bacteria were quantified by qPCR. The effect of contamination on the biodegradation of fatty acid methyl esters (FAMEs) and n-alkanes was assessed by GC/qMS. After 60 days of incubation, B100 and B50 had the highest biomass production at the oil-water interface. Microorganism abundance was estimated by the amplification of 16S rRNA and 18S rRNA genes using qPCR. The fungal population formed at the oil-water interface was larger than the bacterial population, in all treatments. The abundance of bacteria with the alkB gene found in the blends was one or two orders of magnitude higher than that of the total bacteria estimated. The highest biodegradation rates of the C16:0, C18:0, C18:1 cis and C18:2 FAMEs (25.5, 17.5%, 34.5%, and 27.7%, respectively) occurred in the B5 blend. The highest n-alkane degradation occurred in the B50 and B10 blends. The n-alkanes C-20, C-18, C-15, C-14, and C-13 had 72.1%, 57.1%, 18.5%, 21.8%, and 19.6% degradation in B50, respectively. The n-alkanes C19, C12, and C10 had 61.3%, 6.9%, and 22.97% degradation in B10, respectively. The results indicate that knowledge about the microbial contamination of fuels is important for a correct management and to reduce the associated economic losses.
机译：了解微生物群落结构的变化及其在柴油和生物柴油存储过程中的影响，对于防止损坏系统并确保为消费者提供优质产品至关重要。在本研究中，模拟了纯柴油（B0），柴油和生物柴油混合物（B5，B10和B50）以及纯生物柴油（B100）的存储。在模拟过程中以及接种物污染后，通过生物量形成来监测微生物的生长。通过qPCR定量真菌和细菌。通过GC / qMS评估了污染对脂肪酸甲酯（FAME）和正构烷烃生物降解的影响。孵育60天后，B100和B50在油水界面的生物量最高。通过使用qPCR扩增16S rRNA和18S rRNA基因来估计微生物的丰度。在所有处理中，在油水界面形成的真菌种群都大于细菌种群。共混物中发现的带有alkB基因的细菌比估计的细菌总数高出一两个数量级。 B5共混物中C16：0，C18：0，C18：1顺式和C18：2 FAME的最高生物降解率（分别为25.5、17.5％，34.5％和27.7％）。最高的正构烷烃降解发生在B50和B10共混物中。正构烷烃C-20，C-18，C-15，C-14和C-13在B50中的降解率分别为72.1％，57.1％，18.5％，21.8％和19.6％。正构烷烃C19，C12和C10在B10中的降解率分别为61.3％，6.9％和22.97％。结果表明，有关燃料微生物污染的知识对于正确管理和减少相关的经济损失非常重要。

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  • 来源
    《Fuel》 |2018年第1期|911-917|共7页
  • 作者

    Bucker Francielle; de Moura Tiane Martin; da Cunha Michele Espinosa; de Quadros Patricia Dorr; Beker Sabrina Anderson; Cazarolli Juciana Clarice; Caramao Elina Bastos; Guedes Frazzon Ana Paula; Bento Fatima Menezes;

  • 作者单位

    Univ Fed Rio Grande do Sul, Dept Microbiol Immunol & Parasitol, Fuels & Biofuels Biodeteriorat Lab LAB BIO, Sarmento Leite St 500, Porto Alegre, RS, Brazil;

    Univ Fed Rio Grande do Sul, Dept Microbiol Immunol & Parasitol, Fuels & Biofuels Biodeteriorat Lab LAB BIO, Sarmento Leite St 500, Porto Alegre, RS, Brazil;

    Univ Fed Rio Grande do Sul, Dept Inorgan Chem, Inst Chem, Bento Goncalves Ave 9500, Porto Alegre, RS, Brazil;

    Univ Fed Rio Grande do Sul, Dept Microbiol Immunol & Parasitol, Fuels & Biofuels Biodeteriorat Lab LAB BIO, Sarmento Leite St 500, Porto Alegre, RS, Brazil;

    Univ Fed Rio Grande do Sul, Dept Microbiol Immunol & Parasitol, Fuels & Biofuels Biodeteriorat Lab LAB BIO, Sarmento Leite St 500, Porto Alegre, RS, Brazil;

    Univ Fed Rio Grande do Sul, Dept Microbiol Immunol & Parasitol, Fuels & Biofuels Biodeteriorat Lab LAB BIO, Sarmento Leite St 500, Porto Alegre, RS, Brazil;

    Univ Tiradentes, Ind Biotechnol, Aracaju, SE, Brazil;

    Univ Fed Rio Grande do Sul, Dept Microbiol Immunol & Parasitol, Fuels & Biofuels Biodeteriorat Lab LAB BIO, Sarmento Leite St 500, Porto Alegre, RS, Brazil;

    Univ Fed Rio Grande do Sul, Dept Microbiol Immunol & Parasitol, Fuels & Biofuels Biodeteriorat Lab LAB BIO, Sarmento Leite St 500, Porto Alegre, RS, Brazil;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Microbial contamination; Fuel biodeterioration; Alkane-degrading bacteria, GC/qMS; Storage tank;

    机译：微生物污染;燃料生物降解;降解烷烃的细菌;GC / qMS;储罐;

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