• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Fuel >Efficient production of 2,3-butanediol from cheese whey powder (CWP) solution by Klebsiella pneumoniae through integrating pulsed fed-batch fermentation with a two-stage pH control strategy
【24h】

Efficient production of 2,3-butanediol from cheese whey powder (CWP) solution by Klebsiella pneumoniae through integrating pulsed fed-batch fermentation with a two-stage pH control strategy

机译：通过将脉冲补料分批发酵与两阶段pH控制策略相结合，由肺炎克雷伯菌有效地从干酪乳清粉（CWP）溶液中生产2,3-丁二醇

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In this study, several strains were investigated to evaluate their ability to produce 2,3-butanediol (2,3BD) from cheese whey powder (CWP). The capacity of these strains for 2,3-BD production was assessed in lactose medium and two strains Klebsiella pneumoniae CICC 10781 and K. oxytoca CICC 21518 were selected. Fully assessing the fermentation performances indicate that CICC 10781 was more suitable for 2,3-BD production from CWP than CICC 21518. Response surface methodology was applied to optimize the medium composition. Na3C6H5O7.2H(2)O, CH3COONa, and urea were the key factors influencing 2,3-BD synthesis, and the optimal concentrations were 3.65, 5.45, and 0.54 g/L, respectively. Two-stage pH control strategy with pulsed fed-batch fermentation was then developed, in which the pH was maintained at 6.5 for the first 8 h and then shifted to 6.0 till the end of fermentation. Using this strategy, the maximal 2,3-BD production reached 57.63 g/L. The results may provide new guidance for industrial-scale production of 2,3-BD from CWP. (C) 2017 Elsevier Ltd. All rights reserved.
机译：在这项研究中，研究了几种菌株，以评估其从干酪乳清粉（CWP）生产2,3-丁二醇（2,3BD）的能力。在乳糖培养基中评估了这些菌株产生2，3-BD的能力，并选择了两个菌株肺炎克雷伯氏菌CICC 10781和产氧克雷伯氏菌CICC 21518。全面评估发酵性能表明，CICC 10781比CICC 21518更适合于由CWP生产2，3-BD。采用响应面法优化培养基组成。 Na3C6H5O7.2H（2）O，CH3COONa和尿素是影响2,3-BD合成的关键因素，最佳浓度分别为3.65、5.45和0.54 g / L。然后开发了采用间歇补料分批发酵的两阶段pH控制策略，其中在开始的8小时内将pH维持在6.5，然后转移到6.0直至发酵结束。使用该策略，最大的2,3-BD产量达到57.63 g / L。结果可能为CWP工业规模生产2,3-BD提供新的指导。 （C）2017 Elsevier Ltd.保留所有权利。

著录项

  • 来源
    《Fuel》 |2017年第1期|469-477|共9页
  • 作者

    Guo Xuewu; Wang Yazhou; Guo Jian; Wang Qing; Zhang Yu; Chen Yefu; Zhang Cuiying; Xiao Dongguang;

  • 作者单位

    Tianjin Univ Sci & Technol, Coll Biotechnol, Tianjin Ind Microbiol Key Lab, Key Lab Ind Fermentat Microbiol,Minist Educ, Tianjin 300547, Peoples R China|Tianjin Food Safety & Low Carbon Mfg Collaborat I, Tianjin 300457, Peoples R China;

    Tianjin Univ Sci & Technol, Coll Biotechnol, Tianjin Ind Microbiol Key Lab, Key Lab Ind Fermentat Microbiol,Minist Educ, Tianjin 300547, Peoples R China;

    Tianjin Univ Sci & Technol, Coll Biotechnol, Tianjin Ind Microbiol Key Lab, Key Lab Ind Fermentat Microbiol,Minist Educ, Tianjin 300547, Peoples R China;

    Tianjin Univ Sci & Technol, Coll Biotechnol, Tianjin Ind Microbiol Key Lab, Key Lab Ind Fermentat Microbiol,Minist Educ, Tianjin 300547, Peoples R China;

    Tianjin Univ Sci & Technol, Coll Biotechnol, Tianjin Ind Microbiol Key Lab, Key Lab Ind Fermentat Microbiol,Minist Educ, Tianjin 300547, Peoples R China|Tianjin Food Safety & Low Carbon Mfg Collaborat I, Tianjin 300457, Peoples R China;

    Tianjin Univ Sci & Technol, Coll Biotechnol, Tianjin Ind Microbiol Key Lab, Key Lab Ind Fermentat Microbiol,Minist Educ, Tianjin 300547, Peoples R China|Tianjin Food Safety & Low Carbon Mfg Collaborat I, Tianjin 300457, Peoples R China;

    Tianjin Univ Sci & Technol, Coll Biotechnol, Tianjin Ind Microbiol Key Lab, Key Lab Ind Fermentat Microbiol,Minist Educ, Tianjin 300547, Peoples R China|Tianjin Food Safety & Low Carbon Mfg Collaborat I, Tianjin 300457, Peoples R China;

    Tianjin Univ Sci & Technol, Coll Biotechnol, Tianjin Ind Microbiol Key Lab, Key Lab Ind Fermentat Microbiol,Minist Educ, Tianjin 300547, Peoples R China|Tianjin Food Safety & Low Carbon Mfg Collaborat I, Tianjin 300457, Peoples R China;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Cheese whey powder; 2,3-Butanediol; Fermentation; Klebsiella pneumoniae; Two-stage pH control strategy;

    机译：干酪乳清粉;2,3-丁二醇;发酵;肺炎克雷伯菌;两阶段pH控制策略;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号