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Biocatalytic conversion of lipids from microalgae Scenedesmus obliquus to biodiesel using Pseudomonas fluorescens lipase

机译:使用荧光假单胞菌脂肪酶的生物催化转化从斜藻微藻到生物柴油的脂质。

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摘要

Conversion of microalgal lipids using biocatalyst is a novel and greener approach to produce biodiesel. Free and immobilized lipases from Candida sp. and Pseudomonas fluorescens along with free lipases from porcine pancreas and wheat germ were screened for biodiesel conversion of Scenedesmus obliquus lipids. Among selected lipases from various sources immobilized lipase from P. fluorescens showed superior biodiesel conversion. Optimization of reaction parameters viz. lipase amount, temperature, methanol to oil molar ratio and water content was carried out using response surface methodology. Best conversion of 66.55% was achieved at 35 degrees C, methanol to oil ratio of 3:1 with 10% enzyme amount and 2.5% water content based on oil weight. To tackle methanol tolerance step-wise methanol addition was applied, which improved biodiesel conversion upto 90.81%. Immobilized P. fluorescens lipase can be used for 4 batches without much loss in conversion efficiency (>95%). Biodiesel produced has the cetane number of 51.77, Calorific value of 37.67 MJ kg (1). Most of the fuel properties of biodiesel met the specifications set by ASTM and EN standards. (C) 2015 Elsevier Ltd. All rights reserved.
机译:使用生物催化剂转化微藻类脂质是生产生物柴油的一种新颖且绿色的方法。来自假丝酵母的游离和固定化脂肪酶。筛选了猪假单胞菌和荧光假单胞菌,以及来自猪胰腺和小麦胚芽的游离脂肪酶,以进行斜生scenedesmus脂质的生物柴油转化。在来自各种来源的选定脂肪酶中,来自荧光假单胞菌的固定化脂肪酶显示出优异的生物柴油转化率。优化反应参数即。使用响应表面方法进行脂肪酶的量,温度,甲醇与油的摩尔比和水含量。在35摄氏度,甲醇与油的比例为3:1(酶含量为10%,水含量为2.5%,基于油重)的情况下,最佳转化率为66.55%。为了解决甲醇耐受性问题,逐步添加了甲醇,可将生物柴油转化率提高至90.81%。固定化的荧光假单胞菌脂肪酶可用于4批,而转换效率没有很大损失(> 95%)。生产的生物柴油的十六烷值为51.77,发热量为37.67 MJ kg(1)。生物柴油的大多数燃料特性均符合ASTM和EN标准设定的规格。 (C)2015 Elsevier Ltd.保留所有权利。

著录项

  • 来源
    《Fuel》 |2015年第1期|117-124|共8页
  • 作者单位

    Durban Univ Technol, Inst Water & Wastewater Technol, Durban, South Africa;

    Durban Univ Technol, Inst Water & Wastewater Technol, Durban, South Africa;

    Durban Univ Technol, Inst Water & Wastewater Technol, Durban, South Africa;

    Durban Univ Technol, Dept Biotechnol & Food Technol, Durban, South Africa;

    Durban Univ Technol, Inst Water & Wastewater Technol, Durban, South Africa;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Biodiesel; Microalgae; Lipase; Scenedesmus obliquus; Pseudomonas fluorescens;

    机译:生物柴油;微藻;脂肪酶;斜柏目;荧光假单胞菌;

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