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Optimisation of simultaneous saccharification and fermentation of wheat straw for ethanol production

机译：小麦秸秆同时糖化发酵生产乙醇的工艺优化

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摘要

With the goal of obtaining a high ethanol yield, wheat straw (WS) was pretreated with a 1.0% (w/w) NaOH solution. Further parameters, including temperature, enzyme loading, yeast concentration and pH that would affect the ethanol yield through simultaneous saccharification and fermentation (SSF), were optimised by means of Box-Behnken design (BBD), a response surface methodology (RSM). All of the experiments were carried out with an initial solid content of 16.0% (w/v) and fermentation time of 120 h. A well fitted regression equation with an R~2 value of 0.9603 was obtained. The predicted maximum response value (ethanol yield) was 69.49% under the optimised conditions for SSF: temperature of 37.5 ℃, enzyme loading of 30 FPU/g substrate, yeast concentration of 10 g/L and pH of 4.6. The validation experimental result showed that the maximum ethanol yield was 70.76%, which was in accordance with the predicted value.
机译：为了获得高乙醇收率，用1.0％（w / w）的NaOH溶液预处理麦草（WS）。通过Box-Behnken设计（BBD），响应面方法（RSM）优化了其他参数，包括温度，酶负载，酵母浓度和pH，这些参数会通过同时糖化和发酵（SSF）影响乙醇产量。所有实验均以16.0％（w / v）的初始固体含量和120 h的发酵时间进行。得到一个拟合良好的回归方程，R〜2值为0.9603。在SSF的优化条件下预测的最大反应值（乙醇收率）为69.49％，温度为37.5℃，酶负载为30 FPU / g底物，酵母浓度为10 g / L，pH为4.6。验证实验结果表明，最大乙醇产率为70.76％，与预测值一致。

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  • 来源
    《Fuel》 |2013年第10期|331-337|共7页
  • 作者

    Wei Zhang; Yan Lin; Qi Zhang; Xinze Wang; Deyi Wu; Hainan Kong;

  • 作者单位

    School of Environmental Science and Engineering, 800 Dong Chuan Road, Shanghai Jiao Tong University, Shanghai 200240, China;

    School of Environmental Science and Engineering, 800 Dong Chuan Road, Shanghai Jiao Tong University, Shanghai 200240, China;

    School of Environmental Science and Engineering, 800 Dong Chuan Road, Shanghai Jiao Tong University, Shanghai 200240, China;

    School of Environmental Science and Engineering, 800 Dong Chuan Road, Shanghai Jiao Tong University, Shanghai 200240, China;

    School of Environmental Science and Engineering, 800 Dong Chuan Road, Shanghai Jiao Tong University, Shanghai 200240, China;

    School of Environmental Science and Engineering, 800 Dong Chuan Road, Shanghai Jiao Tong University, Shanghai 200240, China;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Ethanol yield; Box-Behnken design (BBD); Response surface methodology (RSM); Simultaneous saccharification and fermentation (SSF); Wheat straw (WS);

    机译：乙醇收率;Box-Behnken设计（BBD）;响应面方法（RSM）;同时糖化和发酵（SSF）;小麦秸秆（WS）;

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