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Thin layer chromatography and image analysis to detect glycerol in biodiesel

机译:薄层色谱和图像分析以检测生物柴油中的甘油

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摘要

Glycerol is a by-product of biodiesel production and is also considered a contaminant in the final product. Current US and EU standards place limits on the permissible levels of free glycerol and bound (unconverted) mono, di and triglycerides in biodiesel, which can be quantified using gas or high pressure liquid chromatography. We report a method based on thin layer chromatography that can be effectively used to determine the presence of glycerol in biodiesel. Visual and machine (image analysis) detection limits of 0.1% and 0.2% v/v of free glycerol, respectively, were established using this procedure. In conjunction with a suitable sample enrichment procedure, this procedure could provide a cost effective alternative to glycerol detection.
机译:甘油是生物柴油生产的副产品,也被认为是最终产品中的污染物。当前的美国和欧盟标准对生物柴油中游离甘油和结合的(未转化的)甘油单酯,甘油二酯和甘油三酯的允许含量进行了限制,可以使用气相色谱法或高压液相色谱法对其进行定量。我们报告了一种基于薄层色谱的方法,该方法可以有效地用于确定生物柴油中甘油的存在。使用此程序分别建立了游离甘油的视觉和机器(图像分析)检测极限,分别为0.1%v / v和0.2%v / v。结合适当的样品富集程序,此程序可以为甘油检测提供经济有效的替代方法。

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