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首页> 外文期刊>Genes & Genomics >Cloning and characterization of Tc1 family-derived PPTN related transposons from ridged-eye flounder (Pleuronichthys cornutus) and inshore hagfish (Eptatretus burgeri)
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Cloning and characterization of Tc1 family-derived PPTN related transposons from ridged-eye flounder (Pleuronichthys cornutus) and inshore hagfish (Eptatretus burgeri)

机译：脊眼比目鱼（Pleuronichthys cornutus）和近岸海鱼（Eptatretus burgeri）Tc1家族与PPTN相关的转座子的克隆和鉴定

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摘要

The Tc1 transposable element is the most widespread family among animal transposon and these elements consist of an inverted repeat (IR) sequence flanking a transposase gene that belongs to Class II type transposon, which is highly conserved in the genome of the nematode C. elegans. In order to characterize Tc1-like transposable elements from several fishes, PPTN (Tc1-like transposon was isolated from Pleuronectes platessa, marine flatfish species) IR primer-specific amplified elements were cloned from the genomic DNA of several fishes. Transposable elements were found in ridged-eye flounder (Pleuronichthys cornutus) and inshore hagfish (Eptatretus burgeri) and named as PCTN and EBTN, respectively. Amino acid sequence alignment and phylogenetic analysis confirmed that the PPTN-like transposons belonged to the Tc1 superfamily of transposons, but they comprised a unique clade of Tc1-like transposons. The IR-PCR analysis using MMTS-IR and PPTN-IR specific primers from Paralichthys olivaceus (Paralichthyidae), Paraplagusia japonica (Cynoglossidae), P. yokohamae (Pleuronectidae) and Pagurus cornutus (Pleuronectidae) (within the same order, Pleuronectiformes but different families) exhibited mutually exclusive distribution of Tc1 family-derived PPTN and MMTS-like transposons in these fish genomes. These results indicate that Tc1 family-derived PPTN and MMTS related Tc1-like transposable elements have uniquely evolved in piscine genome, and can be used as phylogenetic markers for the distribution of subfamilies of Tc1-like transposon and the involvement of horizontal and vertical transmission in the evolution of fish genome.
机译：Tc1转座因子是动物转座子中最广泛的家族，这些元件由位于II类转座子的转座酶基因侧翼的反向重复（IR）序列组成，该基因在线虫秀丽隐杆线虫的基因组中高度保守。为了表征几种鱼类的Tc1样转座因子，从几种鱼类的基因组DNA中克隆了PPTN（从Tleuronectes platesa（海洋比目鱼物种）中分离出Tc1样转座子）的IR引物特异性扩增元件。在脊眼比目鱼（Pleuronichthys cornutus）和近岸海鱼（Eptatretus burgeri）中发现了转座因子，分别命名为PCTN和EBTN。氨基酸序列比对和系统发育分析证实，PPTN样转座子属于转座子的Tc1超家族，但它们包含Tc1样转座子的独特分支。使用oliparaeuthys olivaceus（Paralichthyidae），Japonica（Cynoglossidae），P。yokohamae（Pleuronectidae）和Pagurus cornutus（Pleuronectidae）（相同顺序，但Pleuronectiformes）的MMTS-IR和PPTN-IR特异性引物进行IR-PCR分析）在这些鱼基因组中表现出Tc1家族衍生的PPTN和MMTS样转座子的互斥分布。这些结果表明，Tc1家族的PPTN和MMTS相关的Tc1样转座因子已在鱼类基因组中发生了独特的进化，可作为系统学标记，用于Tc1样转座子亚家族的分布以及水平和垂直传播中的参与。鱼基因组的进化。

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  • 来源
    《Genes & Genomics》 |2013年第1期|47-58|共12页
  • 作者

    Sang Jung Ahn; Ji-Yeon Kim; Moo-Sang Kim; Hyung Ho Lee;

  • 作者单位

    Department of Biotechnology Pukyong National University">(1);

    Department of Embryology Carnegie Institution for Science">(2);

    Department of Biotechnology Pukyong National University">(1);

    Dongseo University and Technische Universität Berlin Joint Algae Laboratory Dongseo University">(3);

    Department of Biotechnology Pukyong National University">(1);

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  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    Transposon; Tc1; PPTN; MMTS; Inverted repeat;

    机译：转座子;Tc1;PPTN;MMTS;反向重复;

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