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Gene identification using rice genome sequences

机译：利用水稻基因组序列进行基因鉴定

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摘要

Identifying useful gene(s) is one of the most important objectives of plant geneticists. Various strategies can be used, which are based on the characteristics of plant reproduction and available technology. Rice is the first model crop whose whole genome sequence has been reported. In addition, information on the whole genome sequences of two important rice subspecies (japonica and indica rice) is also available. Rice is a self-pollinating crop and methods of artificial crossing are relatively easy to perform; such methods enable the production of numerous seeds for genetic analyses. Based on these features, a map-based cloning (i.e., positional cloning) strategy has been successfully applied over the last decade to identify rice genes. Recently, advanced next-generation sequencing (NGS) technology was used to ascertain the genome sequences of individual plants, opening up a new strategy for gene identification. This strategy has been used successfully to identify the genes responsible for certain qualitative traits in rice. However, to identify the gene(s) involved in a quantitative trait, a map-based cloning strategy is still required after quantitative trait loci analysis using NGS technology. In this review, we discuss both map-based cloning (which is still the primary strategy used to identify rice genes) and NGS-based strategies.
机译：鉴定有用的基因是植物遗传学家最重要的目标之一。可以使用各种策略，这些策略基于植物繁殖的特征和可用技术。水稻是第一个报道了全基因组序列的典型作物。此外，还可以获得有关两个重要水稻亚种（粳稻和in稻）的全基因组序列的信息。水稻是一种自花授粉作物，人工杂交方法相对容易实施。这样的方法能够产生大量用于遗传分析的种子。基于这些特征，在过去十年中已经成功地应用了基于图的克隆（即位置克隆）策略来鉴定水稻基因。最近，先进的下一代测序（NGS）技术用于确定单个植物的基因组序列，从而为基因鉴定开辟了新的策略。该策略已成功用于鉴定水稻中某些定性性状的基因。但是，为了鉴定涉及定量性状的基因，在使用NGS技术进行定量性状基因座分析之后，仍然需要基于图谱的克隆策略。在这篇综述中，我们讨论了基于图的克隆（这仍然是用于鉴定水稻基因的主要策略）和基于NGS的策略。

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  • 来源
    《Genes & Genomics》 |2013年第4期|415-424|共10页
  • 作者

    Joohyun Lee; Hee-Jong Koh;

  • 作者单位

    Department of Applied Bioscience Konkuk University">(1);

    Department of Plant Science Research Institute of Agriculture and Life Sciences Seoul National University">(2);

    Plant Genomics and Breeding Institute Seoul National University">(3);

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Rice map-based cloning; NGS; QTL;

    机译：基于水稻图的克隆;NGS;QTL;

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