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首页> 外文期刊>Genetic Resources and Crop Evolution >Comparing costs for different conservation strategies of garlic (Allium sativum L.) germplasm in genebanks
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Comparing costs for different conservation strategies of garlic (Allium sativum L.) germplasm in genebanks

机译：种质库中大蒜（Alium sativum L.）种质资源不同保存策略的成本比较

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摘要

The maintenance of plant genetic resources in living plant collections (genebanks) causes costs due to employment of staff, usage of buildings, equipment and consumables. Since this is especially challenging in vegetatively propagated material, studies were performed for the case of garlic, which is one of the major vegetatively maintained crops in the genebank of IPK Gatersleben. Data were recorded to compare various scenarios of the main strategies field maintenance and cryopreservation. A spreadsheet tool was developed to be used for cost assessment and for drawing conclusions concerning the most effective way of maintenance. Field culture is cheaper in the short term, whereas after a break-even point cryopreservation becomes the more efficient storage method in the long term. This break-even point depends on the particular scenario, which is determined by various factors such as field and in vitro multiplication rates of various genotypes, presence of bulbils in a part of the genepool, the sample size of the accessions as well as the number of stored accessions in cryopreservation. The comparative discussion is exemplified for a 1-year field rotation versus cryopreservation using either in vitro plantlets or a combination of bulbils and unripe inflorescence bases as organ sources. For the more expensive use of in vitro plants cryopreservation becomes less costly than field culture only after 13years, whereas this is the case already after 8–9years when using a combination of bulbils in winter and inflorescence bases in summer.
机译：活植物集合（基因库）中植物遗传资源的维护由于工作人员的雇用，建筑物，设备和消耗品的使用而产生成本。由于这在营养繁殖材料中特别具有挑战性，因此针对IPK Gatersleben基因库中主要的营养维持作物之一大蒜进行了研究。记录数据以比较现场维护和低温保存主要策略的各种情况。开发了一种电子表格工具，可用于成本评估和得出有关最有效维护方式的结论。在短期内，田间培养更便宜，而在达到收支平衡点后，长期而言，冷冻保存将成为更有效的存储方法。收支平衡点取决于具体情况，具体情况取决于各种因素，例如各种基因型的田间和体外繁殖率，基因池一部分中的子茎存在，种质的样本量以及数量冷冻保存的种质。比较讨论的例子是使用体外小植株或鳞茎和未成熟花序基部的组合作为器官源，进行一年轮作与低温保存。对于更昂贵的体外植物使用，仅在13年后冷冻保存就比田间培养便宜，而在8–9年后，冬季使用鳞茎和夏季使用花序基团时，情况已经如此。

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