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Genetic diversity of carrot (Daucus carota L.) cultivars revealed by analysis of SSR loci

机译:通过SSR基因座分析揭示胡萝卜(Daucus carota L.)品种的遗传多样性

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摘要

Polymorphism of simple sequence repeat (SSR) loci was assessed in a collection of 88 carrot (Daucus carota L. subsp. sativus Hoffm.) accessions. The collection comprised cultivars and landraces mainly from Asia, Europe, and North America. Plants were grown in the glasshouse and characterized for root color and shape. Thirty SSR loci were fully characterized using parameters derived from allele frequencies, i.e. the number of total, effective and rare alleles, the observed and expected heterozygosity, and fixation index. Using a Bayesian approach, two clusters of 17 and 61 accessions were distinguished, which comprised the Asian and Western type accessions, respectively. Genetic diversity of the Asian gene pool was higher than that of the Western gene pool. The results of SSR analysis were supported by morphological characterization, and are congruent with current knowledge on the history of carrot domestication and breeding.
机译:在88个胡萝卜(Daucus carota L. subsp。sativus Hoffm。)品种的集合中评估了简单序列重复(SSR)基因座的多态性。该馆藏主要来自亚洲,欧洲和北美的品种和地方品种。植物在温室中生长,并具有根部颜色和形状的特征。使用源自等位基因频率的参数(即总,有效和稀有等位基因的数量,观察到的和预期的杂合性以及固定指数)充分表征了30个SSR基因座。使用贝叶斯方法,区分了17个和61个种质的两个簇,分别包括亚洲和西方类型的种质。亚洲基因库的遗传多样性高于西方基因库。 SSR分析的结果得到形态学表征的支持,并与当前有关胡萝卜驯化和育种历史的知识相一致。

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