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Characterization of Italian lentil (Lens culinaris Medik.) germplasm by agronomic traits, biochemical and molecular markers

机译:农艺性状,生化和分子标记物表征意大利小扁豆(Lens culinaris Medik。)种质

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摘要

Genetic relationships, agronomic, nutritional and technological traits of ten Italian landraces, two improved lines and two cultivars of lentil (Lens culinaris Medik.) were investigated using a multi-disciplinary approach. Seed storage proteins, used as biochemical markers, were able to detect polymorphisms with variability mainly related to the polypeptide abundance. Microsatellite (SSR) molecular markers provided very useful information on genetic variation and relationships among landraces, with polymorphic fragments able to discriminate all the accessions. Lentil landraces were grouped in different clusters and sub-clusters principally on the basis of their geographical origin. The highest levels of genetic diversity were observed for lentils from ‘Castelluccio di Norcia’, ‘Colliano’ and ‘Villalba’. Field trials, performed in two locations of Southern Italy, revealed a high influence of location on yield. Comparing performances at both tested locations, the best landraces were ‘Linosa’ and ‘Valle di Nevola’ suggesting that these have the highest adaptability. Technological and nutritional data together with the agronomic ones evidenced that ‘Linosa’ lentil is the best landrace, however also ‘San Gerardo’ deserves some attention.
机译:使用多学科方法研究了十个意大利地方品种,两个改良品系和两个扁豆品种(Lens culinaris Medik。)的遗传关系,农艺,营养和技术特性。用作生物化学标记的种子储存蛋白能够检测多态性,其变异性主要与多肽丰度有关。微卫星(SSR)分子标记提供了有关遗传变异和地方品种之间关系的非常有用的信息,其多态性片段能够区分所有种质。扁豆的地方品种主要根据其地理起源分为不同的集群和子集群。观察到来自“ Castelluccio di Norcia”,“ Colliano”和“ Villalba”的小扁豆的遗传多样性最高。在意大利南部的两个地点进行的田间试验表明,地点对产量的影响很大。比较两个测试地点的性能,最好的地方是“ Linosa”和“ Valle di Nevola”,这表明它们具有最高的适应性。技术和营养数据以及农艺数据证明,“ Linosa”小扁豆是最好的地方品种,但是“ San Gerardo”也应引起注意。

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