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首页> 外文期刊>Genetic Resources and Crop Evolution >Identification of sex-specific DNA markers in betel vine (Piper betle L.)
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Identification of sex-specific DNA markers in betel vine (Piper betle L.)

机译：槟榔（Piper betle L.）中性别特异性DNA标记的鉴定

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The Random Amplified Polymorphic DNA (RAPD) technique was used to amplify DNA segments, with the objective of finding markers linked to sex determination in male and female plants of Piper betle L. Two bulks of DNA were made drawing one each from male and female, by pooling an equal volume of DNA samples from each group of individual contributing to the bulk analysis. Fifty different random decamer primers were screened with the two bulks to identify markers associated with sex expression of which only four primers were found to be associated with sex expression. These four primers were then tested with individual plant DNA samples where sex-associated RAPD markers were identified. A ~1,400 and ~850 bp fragment from the primer OPA04 and OPN 02 respectively was found to be present in all the male individuals and absent in all the female plants. In another primer, a ~980 bp amplification product from the primer OPC 06 was present only in the female individuals. A common primer OPA 08 showed both male and female specific markers of 650 and 1,200 bp respectively. Thus, the three male- specific RAPD markers OPA041400, OPA08650 and OPN02850 and two female-specific markers OPA081200 and OPC06980 can reliably differentiate the male and female plants of P. betle L. Ploidy comparison also showed the differences in male and female plants.
机译：随机扩增多态DNA（RAPD）技术用于扩增DNA片段，目的是寻找与Piper betle L的雄性和雌性植物性别决定相关的标记。从雄性和雌性中分别提取两束DNA，通过汇集来自每组个体的等体积的DNA样品，有助于整体分析。用两个主体筛选了五十种不同的随机十聚体引物，以鉴定与性别表达相关的标记，其中仅发现四个与性别表达相关的引物。然后，用鉴定出性别相关的RAPD标记的单个植物DNA样品测试这四种引物。发现来自引物OPA04和OPN 02的〜1,400和〜850bp片段分别存在于所有雄性个体中，而在所有雌性植物中均不存在。在另一个引物中，来自引物OPC 06的〜980 bp扩增产物仅存在于雌性个体中。通用引物OPA 08分别显示男性和女性特异性标记，分别为650和1200 bp。因此，三个雄性特异性RAPD标记OPA041400 ，OPA08650 和OPN02850 以及两个雌性特异性标记OPA081200 和OPC06980 可以可靠地区分雄性和雌性甲壳虫的L.倍性的比较还显示出雌雄植物的差异。

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