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Storage protein and amino acid contents of Tunisian and Chinese pomegranate (Punica granatum L.) cultivars

机译:突尼斯和石榴(Punica granatum L.)品种的贮藏蛋白和氨基酸含量

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摘要

In the present study, free amino acids and seed protein contents of pomegranate germplasm from Tunisia and China were conducted using Hitachi L8800 amino acid analyzer, Bradford and Kjeldahl methods. The work offers more consideration to protein pomegranate seeds which can be with great importance regarding the nutritive seed value of this crop. In other hand, the protein and amino acids contents were used to compare the Tunisian and Chinese pomegranate cultivars based on multivariate analyses (principal component and clustering analyses). Results show that the Tunisian and Chinese pomegranate seeds were rich in storage proteins independently of organoleptic taste as sweet, sour sweet or sour. Qualitatively, the storage protein and free amino acids of pomegranate seeds were identical. Besides, the segregation of Chinese and Tunisian pomegranate cultivars into three groups was reveled based on high, low or moderate amino acids contents. Statistically, the differences between organoleptic groups (sweet, sour sweet and sour) were not significant (P 0.05). However, the differences between pomegranate cultivars were statistically significant within groups. The separation of cultivars is independent to the organoleptic taste neither the geographical origin.
机译:在本研究中,使用日立L8800氨基酸分析仪,Bradford和Kjeldahl方法进行了突尼斯和中国石榴种质的游离氨基酸和种子蛋白含量的测定。这项工作为石榴蛋白种子提供了更多考虑,这对于该作物的营养种子价值可能非常重要。另一方面,基于多变量分析(主要成分和聚类分析),使用蛋白质和氨基酸含量比较突尼斯和中国石榴品种。结果表明,突尼斯和中国石榴种子均富含贮藏蛋白,而与感官口味(甜,酸甜或酸)无关。定性地,石榴籽的贮藏蛋白和游离氨基酸相同。此外,基于高,低或中等氨基酸含量,揭示了中国和突尼斯石榴品种的分成三类。统计学上,感官组之间的差异(甜味,酸甜味和酸味)不显着(P <0.05)。但是,石榴品种之间的差异在各组之间具有统计学意义。品种的分离与感官口味无关,也与地理来源无关。

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  • 来源
    《Genetic Resources and Crop Evolution》 |2012年第6期|p.999-1014|共16页
  • 作者单位

    Laboratoire d’Aridoculture et Cultures Oasiennes, Institut des Régions Arides de Médenine, 4119, Medenine, Tunisia;

    Institut Supérieur des Sciences Appliquées et de Technologie, 6072, Gabès, Tunisia;

    Laboratory of Plant Biotechnology, National Institute of Applied Sciences and Technology, B.P. 676, 1080, Tunis Cedex, Tunisia;

    Laboratoire de Biochimie, Département de Biologie, Faculté des Sciences de Tunis, Université Tunis El-Manar, 2092, Tunis, Tunisia;

    Laboratoire d’Aridoculture et Cultures Oasiennes, Institut des Régions Arides de Médenine, 4119, Medenine, Tunisia;

    Laboratoire d’Aridoculture et Cultures Oasiennes, Institut des Régions Arides de Médenine, 4119, Medenine, Tunisia;

    School of Food Science and Engineering, Harbin Institute of Technology, Harbin, 150090, China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Clustering analysis; Free Amino acids; PCA; Pomegranate seed; Punica granatum L.; Storage protein;

    机译:聚类分析游离氨基酸PCA石榴籽石榴贮藏蛋白;

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