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Effects of domestication on structural polysaccharides and dietary fiber in nopalitos (Opuntia spp.)

机译:驯化对Nopalitos(Opuntia spp。)结构多糖和膳食纤维的影响

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Domestication is an evolutionary process that modifies morphological, physiological, chemical and genetic features of wild plants, and is a product of artificial selection. A gradient of domestication can be appreciated in species of the Opuntia genus. There are wild species like O. streptacantha, others semi-domesticated like O. hyptiacantha O. megacantha and O. albicarpa, or other like O. ficus-indica with the highest degree of domestication. At the same time, some structural polysaccharides have been associated with plant drought and freeze resistance. This study aimed at quantify five groups of structural polysaccharides in 14 variants of nopalitos (edible young cladodes of flat-stemmed spiny cacti consumed as vegetables) of Opuntia spp. in a domestication gradient. Given that extreme environment is less frequent under cultivation than in the wild, we hypothesized that structural polysaccharides in nopalitos are reduced throughout the domestication process. The experiment was conducted as a completely randomized design with a 5 × 14 two-factor factorial treatment structure, with four replications. Mucilages, pectins, hemicelluloses and cellulose were extracted and dietary fiber content was calculated. ANOVA of data was performed, pair wise comparisons of species and variants were conducted by Tukey test and principal components analysis was carried out. Mucilages were higher in O. ficus-indica (12% of total dry mass) and pectins, loosely and tightly bound hemicelluloses in O. streptacantha (2.5, 9 and 3%, respectively). All five species had similar content of cellulose (5.1% dry mass); but, O. streptacantha presented more dietary fiber. Higher pectins and both loosely and tightly bound hemicelluloses content in wild species can be related to genotype and the persistence of reaction to a wild environment. Mucilages which are one of the main characteristics of consumer preference and loosely bound hemicelluloses could have impact for selection during Opuntia domestication.
机译:驯化是一种进化过程,可以改变野生植物的形态,生理,化学和遗传特征,是人工选择的产物。在仙人掌属的物种中可以理解驯化的梯度。有野生物种,如链霉菌(O. streptacantha),其他半驯化物种(如O. hyptiacantha O. megacantha和O. albicarpa),或其他像驯化程度最高的O. ficus-indica。同时,一些结构性多糖与植物的干旱和抗冻性有关。这项研究旨在量化仙人掌属物种的nopalitos(食用植物的扁平茎多刺仙人掌的可食用的年轻枝状复合体)的14个变体中的五种结构多糖。在驯化梯度中。鉴于极端环境下耕种的情况比野生环境下少,因此我们假设胭脂虫中的结构多糖在整个驯化过程中都会减少。该实验以完全随机的设计进行,具有5×14的两因素析因处理结构,具有四次重复。提取粘液​​,果胶,半纤维素和纤维素,并计算膳食纤维含量。进行数据的ANOVA,通过Tukey测试进行物种和变体的成对比较,并进行主成分分析。 fi稻(O. ficus-indica)(占总干重的12%)和果胶中的粘液含量较高,链霉菌(O. streptacantha)中的半纤维素松散且紧密结合(分别为2.5%,9%和3%)。所有五个种类的纤维素含量相似(干质量为5.1%);但是,链霉菌的膳食纤维含量更高。果胶中较高的果胶以及松散和紧密结合的半纤维素含量都可能与基因型和对野生环境的反应持续存在有关。黏液是消费者偏好的主要特征之一,并且半纤维素的松散结合可能会影响其在驯化仙人掌期间的选择。

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