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首页> 外文期刊>Genetic Resources and Crop Evolution >Molecular characterization of four HMW glutenin genes from Heteranthelium piliferum C. E. Hubbard and Henrardia persica (Banks et Solander) Hochstetter
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Molecular characterization of four HMW glutenin genes from Heteranthelium piliferum C. E. Hubbard and Henrardia persica (Banks et Solander) Hochstetter

机译：四个杂种的HMW谷蛋白基因的分子特征：杂种C. E. Hubbard和Henrardia persica（Banks et Solander）Hochstetter

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摘要

High molecular weight (HMW) glutenin genes Qy and Oy were isolated and characterized. Compared with previously reported HMW genes, they showed modifications in all domains. Qy genes had an extra glutamine and 105 residues in the N terminal, two irregular tandem repeats of P–GQQGQQVYYPTAPQQ in the repetitive domain, and had the same short peptide LAAQLPAMCRLE as in x-type genes in the C-terminal. Besides these modifications, the 11 aa peptides, GSYYPGQASPQQ, conserved in the repetitive domain in all genes, were absent in Qy. Oy genes with 103 residues, had the short IPTLLS peptide found in x-type genes in the N terminal, and had unique tandem repeats of PGQGQ-RQQGYYPTSQQQ and GQQGQQ in the repetitive domain, and shared the short peptide LAAQLPAMCRLE with those of x-type genes in the C-terminal. Three of four y gene ORFs were successfully expressed in bacteria. In the phylogenetic tree based on the N- and C-terminal sequences, Qy and Oy genes clustered with y and x genes, respectively. This suggested that genes Qy and Oy were not pure y genes, but also had some characteristics of x genes. These unique structures provide new information for understanding the origin and evolution of x and y genes as well as wheat quality improvement.
机译：高分子量（HMW）谷蛋白基因Qy和Oy进行了分离和表征。与以前报道的HMW基因相比，它们在所有域均显示出修饰。 Qy基因在N末端有一个额外的谷氨酰胺和105个残基，在重复域中有两个不规则的P–GQQGQQVYYPTAPQQ串联重复序列，在C末端具有与x型基因相同的短肽LAAQLPAMCRLE。除这些修饰外，所有基因的重复结构域中均保守的11个氨基酸的GSYYPGQASPQQ氨基酸在Qy中不存在。具有103个残基的Oy基因在N末端的x型基因中具有短IPTLLS肽，并且在重复域中具有PGQGQ-RQQGYYPTSQQQ和GQQGQQ的独特串联重复序列，并且与x型基因共享短肽LAAQLPAMCRLE C末端的基因。四个y基因ORF中的三个已在细菌中成功表达。在基于N端和C端序列的系统树中，Qy和Oy基因分别与y和x基因成簇。这表明基因Qy和Oy不是纯y基因，但也具有x基因的某些特征。这些独特的结构为了解x和y基因的起源和进化以及小麦品质的提高提供了新的信息。

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  • 来源
    《Genetic Resources and Crop Evolution》 |2012年第7期|p.1309-1318|共10页
  • 作者

    Xiang-Quan Wang; Ze-Hong Yan; Shou-Fen Dai; Deng-Cai Liu; Yu-Ming Wei; You-Liang Zheng;

  • 作者单位

    Triticeae Research Institute, Sichuan Agricultural University, Wenjiang District, No. 555 Northeast Road, Chengdu City, 611130, Sichuan, China;

    Triticeae Research Institute, Sichuan Agricultural University, Wenjiang District, No. 555 Northeast Road, Chengdu City, 611130, Sichuan, China;

    Triticeae Research Institute, Sichuan Agricultural University, Wenjiang District, No. 555 Northeast Road, Chengdu City, 611130, Sichuan, China;

    Triticeae Research Institute, Sichuan Agricultural University, Wenjiang District, No. 555 Northeast Road, Chengdu City, 611130, Sichuan, China;

    Triticeae Research Institute, Sichuan Agricultural University, Wenjiang District, No. 555 Northeast Road, Chengdu City, 611130, Sichuan, China;

    Triticeae Research Institute, Sichuan Agricultural University, Wenjiang District, No. 555 Northeast Road, Chengdu City, 611130, Sichuan, China;

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  • 正文语种 eng
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  • 关键词

    Henrardia persica; Heteranthelium piliferum; HMW glutenin subunit; SDS-PAGE; Sequence analysis;

    机译：百日咳;猪上皮;HMW谷蛋白亚基;SDS-PAGE;序列分析;

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