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首页> 外文期刊>Genetic Resources and Crop Evolution >Re-sequencing of vrs1 and int-c loci shows that labile barleys (Hordeum vulgare convar. labile) have a six-rowed genetic background
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Re-sequencing of vrs1 and int-c loci shows that labile barleys (Hordeum vulgare convar. labile) have a six-rowed genetic background

机译：vrs1和int-c基因座的重测序表明不稳定的大麦（大麦变种不育）具有六行遗传背景

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Labile-barleys (Hordeum vulgare convar. labile (Schiem.) Mansf.) are found in the highlands of Ethiopia, Eretria and North India-Pakistan districts. They represent a distinct spike form showing row-type alterations even within individual spikes of the same genotypes. Variation at the six-rowed spike 1 (vrs1) locus is sufficient to control barley lateral spikelet fertility, which is also modified by alleles at the intermedium-c (int-c) locus. This study aimed at re-sequencing these two loci to investigate whether labile-barleys have a two-rowed genetic background, resulting in increased lateral spikelet fertility, or show reduced lateral spikelet fertility if they possess a six-rowed genetic background. The Vrs1 re-sequencing results of 221 supposedly labile-barley accessions from Ethiopia revealed 13 accessions with two novel vrs1.a1 haplotypes. Following the current nomenclature of vrs1 haplotypes, the new haplotypes were named as haplotypes 66 and 67. Re-sequencing at the int-c locus showed that 118 of the labile-barleys possessed the previously described Int-c.a allele but only one accession was found having a novel Int-c.a haplotype in the homozygous state (termed Int-c.a haplotype1; Hap_1). Interestingly, 101 labile-barleys carried the Int-c.a allele and Int-c.a haplotype1 simultaneously, suggesting maintained heterozygosity or recent gene duplication at this locus. Only one accession had a two-rowed haplotype (Vrs1.b3, int-c.b1) and one accession possessed the Vrs1.t (deficiens) and Int-c.a alleles (six-rowed). These two accessions were considered as misclassified labile genotypes and not included in further analysis. Thus, these results confirmed that all of the 219 labile accessions studied in this work showed six-rowed alleles at vrs1 but reduced lateral spikelet fertility. This reduction is most likely caused by the recessive labile (lab) locus which we are in the process to characterize further.
机译：在埃塞俄比亚，埃雷特里亚和北印度-巴基斯坦地区的高地上发现了大麦（大麦）（大麦）。它们代表了独特的尖峰形式，即使在相同基因型的单个尖峰中也显示行类型的变化。六行穗1（vrs1）位点的变异足以控制大麦侧小穗的受精能力，这也被中度c（int-c）位点的等位基因修饰。这项研究旨在对这两个基因座进行重测序，以研究不稳定的大麦是否具有两行遗传背景，从而导致侧生小穗繁殖力增加，或者显示具有六行遗传背景的侧生小穗繁殖力降低。来自埃塞俄比亚的221个据称不稳定大麦种质的Vrs1重测序结果显示，有13个种质带有两个新颖的vrs1.a1单倍型。根据当前的vrs1单倍型命名法，新的单倍型分别命名为66和67单倍型。在int-c位点进行重测序显示，有118个不稳定大麦具有先前描述的Int-ca等位基因，但仅找到一个登录号。具有纯合子状态的新型Int-ca单倍型（称为Int-ca haplotype1； Hap_1）。有趣的是，101个不稳定的大麦同时携带Int-c。a等位基因和Int-c.a haplotype1，表明在该基因座处保持杂合性或最近的基因重复。只有一个品系具有两行单倍型（Vrs1.b3，int-c.b1），并且一个品系具有Vrs1.t（缺陷）和Int-c.a等位基因（六行）。这两个登录号被认为是错误分类的不稳定基因型，未包括在进一步分析中。因此，这些结果证实，在这项工作中研究的所有219个不稳定种质均在vrs1处显示了六行等位基因，但降低了侧生小穗的繁殖力。这种减少很可能是由隐性不稳定（实验室）基因座引起的，我们正在对其进行进一步表征。

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