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首页> 外文期刊>Genetic Resources and Crop Evolution >Hybridization barriers between diploid Solanum tuberosum and wild Solanum raphanifolium
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Hybridization barriers between diploid Solanum tuberosum and wild Solanum raphanifolium

机译：二倍体马铃薯与野生茄子的杂交障碍

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摘要

Wild potato germplasm represents a unique, diverse and accessible resource for disease and pest resistance, along with useful agronomic traits that may be introgressed into the cultivated potato (Solanum tuberosum L.). Hybridization of diploid wild Solanum species with haploids (2×) of cultivated potato (4×) is generally an effective technique for introducing genetic diversity and desirable traits into potato. However, in this study, hybridization barriers were found in crosses between S. tuberosum haploids and the wild species S. raphanifolium. Male sterility, likely due to nuclear-cytoplasmic interactions, was observed in some haploid tbr × S. raphanifolium hybrids. In addition, pollen-pistil incompatibilities were observed in backcross, F2, and reciprocal cross hybridization attempts that failed to produce seed. More crosses were successful when F1 clones were crossed as females to wild clones than to cultivated clones. When crosses were made in the other direction, with F1 hybrids used as male parents, seeds were almost never produced.
机译：野生马铃薯种质代表着独特的，多样化的和易获得的抗病虫害资源，以及有用的农艺性状，可能会渗入栽培马铃薯（Solanum tuberosum L.）。将二倍体野生茄属物种与栽培马铃薯的单倍体（2x）（4x）杂交通常是将遗传多样性和所需性状引入马铃薯的有效技术。然而，在这项研究中，在马铃薯单倍体单倍体和野生物种雷氏沙门氏菌之间的杂交中发现了杂交障碍。在一些单倍体tbr×S. raphanifolium杂种中观察到雄性不育，可能是由于核质相互作用。此外，在回交，F2和相互交叉杂交尝试均未产生种子的情况下，发现花粉雌蕊不相容。当F1克隆作为雌性与野生克隆杂交时，成功杂交的次数超过了栽培克隆。当以另一个方向杂交时，使用F1杂种作为雄性亲本，几乎从未产生种子。

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