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Genetic and phenotypic diversity in a germplasm working collection of cultivated tropical yams (Dioscorea spp.)

机译:栽培热带山药种质资源集合中的遗传和表型多样性(Dioscorea spp。)

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A working collection of yam (Dioscorea spp.) comprising 53 landraces and seven improved cultivars of four species (D. alata L., D. cayenensis Lam., D. dumetorum (Kunth) and D. rotundata Poir.) was evaluated for phenotypic and genetic diversity. The evaluation involved field assessment of 24 morphological traits and DNA analysis with 32 Simple sequence repeat (SSR) polymorphic markers. Diversity was greater between species than within species; highest in D. rotundata and lowest in D. alata and D. cayenensis. Analysis based on combined phenotypic and SSR marker data sets revealed a close relationship between D. rotundata and D. cayenensis, but D. alata and D. dumetorum remained as distinct species. D. alata was related genetically to D. rotundata and D. cayenensis, but phenotypically to D. dumetorum. The study showed that cultivars obtained from different farmers may bear the same name but be genetically different. Polymorphic SSR markers were identified that may be used for genetic analysis across yam species. The working collection assessed in this study represents a good gene pool for intra- and inter-specific hybridization in yam genetic improvement.
机译:对山药(Dioscorea spp。)的工作集合进行了表型评估,其中包括53种地方品种和7种改良的四个品种(D. alata L.,D。cayenensis Lam。,D。dumetorum(Kunth)和D. rotundata Poir。)。和遗传多样性。评估涉及24种形态特征的现场评估和32种简单重复序列(SSR)多态性标记的DNA分析。物种之间的多样性大于物种内部的多样性; D. rotundata中最高,而D. alata和D. cayenensis中最低。基于组合的表型和SSR标记数据集的分析揭示了D. rotundata和D. cayenensis之间的密切关系,但D. alata和D. dumetorum仍然是不同的物种。 D. alata与D. rotundata和D. cayenensis在遗传上相关,但在表型上与D. dumetorum相关。研究表明,从不同农民那里获得的品种可能具有相同的名称,但在遗传上却有所不同。确定了多态性SSR标记,可用于跨山药物种的遗传分析。在这项研究中评估的工作集合代表了山药遗传改良中种内和种间杂交的良好基因库。

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