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Chinese Cornus officinalis: genetic resources, genetic diversity and core collection

机译：山茱off：遗传资源，遗传多样性和核心收藏

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Cornus officinalis is plant species that is widely used in traditional Chinese medicine. To help the efficient utilization and conservation of this species, the genetic diversity of 73 germplasm resources collected from Zhejiang, Henan and Shaanxi provinces in China were used in a primary core collection. The germplasm resources were classified nine types and the analysis of morphological traits and chemical components showed wide variations among the germplasm types. The primary core collection of C. officinalis was evaluated by using 18 inter-simple sequence repeat (ISSR) markers and a mini core collection was developed based on three strategies on construction of a core germplasm resources. The ISSR results showed that a total of 233 alleles were identified in these 73 accessions, the genetic diversity (P = 91.02%, H = 0.293, I = 0.446) revealed with an average of 2 alleles per locus. The genetic diversity index analysis suggested that there is a great richness and uniqueness of genetic variation in this primary core collection. Cluster analysis by UPGMA and STRUCTURE placed the 73-germplasm resources into four linkage clusters. Almost all samples from one sampling location were clustered together. Compared to the RS and SW strategy, the LDSS strategy proved to be more representative in the core collection of C. officinalis. Finally, a mini core collections by LDSS, composed of 18 accessions (24.66% of the primary core collection), let more than 94.85% of polymorphic loci remained, 97.55% of the observed number of alleles and 99.28% of the effective number of alleles of the mini core collection, respectively. The Nei’s gene diversity and Shannon’s Information index of core collection were 0.294 and 0.443, respectively. Taken all together, the results indicated that mini core collections constructed by the LDSS strategy represented their primary core collections and provided a rational framework for intensive surveys of natural variation in C. officinalis genetic resources.
机译：山茱off是在传统中药中广泛使用的植物。为了帮助该物种的有效利用和保护，从中国浙江，河南和陕西等省收集到的73种种质资源的遗传多样性被用作主要核心种质。种质资源分类为9种，形态特征和化学成分分析表明种质资源之间存在较大差异。通过使用18个简单序列间重复序列（ISSR）标记评估了C. officinalis的主要核心资源，并基于构建核心种质资源的三种策略开发了微型核心资源。 ISSR结果表明，在这73个种质中共鉴定出233个等位基因，遗传多样性（P = 91.02％，H = 0.293，I = 0.446）显示每个基因座平均2个等位基因。遗传多样性指数分析表明，该主要核心收藏物中遗传变异具有极大的丰富性和独特性。 UPGMA和STRUCTURE进行的聚类分析将73种种质资源分为四个连锁聚类。来自一个采样位置的几乎所有样本都聚在一起。与RS和SW策略相比，LDSS策略在C. officinalis的核心收藏中被证明更具代表性。最后，LDSS的微型核心馆藏由18个种质组成（占主要核心馆藏的24.66％），保留了超过94.85％的多态位点，观察到的等位基因数量占97.55％，有效等位基因数量占99.28％。迷你核心系列的分别。 Nei的基因多样性和Shannon的核心收藏信息指数分别为0.294和0.443。综上所述，结果表明，由LDSS策略构建的微型核心馆藏代表了它们的主要核心馆藏，并为深入调查C. officinalis遗传资源的自然变异提供了合理的框架。

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