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Genetic differentiation and diversity of phenotypic characters in Chinese wild soybean (Glycine soja Sieb. et Zucc.) revealed by nuclear SSR markers and the implication for intraspecies phylogenic relationship of characters

机译:核SSR标记揭示中国野生大豆表型性状的遗传分化和多样性及其对种内种系关系的启示

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摘要

Genetic diversity is reduced from wild soybean to cultivars and from landraces to modern varieties. However, intraspecies genetic diversity loss between characters or phenotypes also existed in wild soybean. We revealed the phylogenic relationship of character types in Chinese wild soybean using 42 SSR markers. We conjectured that white flower, no-seed bloom, grey pubescence, and four seed coat colours were evolutionarily acquired phenotypes. There were a small decrease (∆H = 0.1–6.46%) of gene diversity and a moderate reduction (∆Na = 10.81–53.54%) of number of alleles but a violent loss (80.74–98.59%) of unique alleles in the acquired phenotypes. Our results seemed to suggest that ovoid and elliptic leaves were differentiated at the earliest and subsequently lanceolate leaf appeared before the domestication of soybeans within wild soybean, and that G. gracilis type was another earliest type, maybe emerged since the appearance of soybeans or it was concomitant with the domestication of soybean.
机译:从野生大豆到品种,从地方品种到现代品种,遗传多样性都在减少。然而,野生大豆中也存在性状或表型间种内遗传多样性的丧失。我们利用42个SSR标记揭示了中国野生大豆性状类型的系统发生关系。我们推测白花,无籽绽放,灰色短柔毛和四种种皮颜色是进化获得的表型。获得的基因多样性稍有下降(ΔH= 0.1–6.46%),等位基因数目有中等下降(ΔNa= 10.81–53.54%),但获得的独特等位基因发生剧烈损失(80.74–98.59%)表型。我们的结果似乎表明卵形和椭圆形叶片最早分化,随后披针形叶片出现在野生大豆中驯化大豆之前,而G. gracilis类型是最早的类型,可能是由于大豆的出现或伴随大豆的驯化。

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