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首页> 外文期刊>Genetic Resources and Crop Evolution >Characterization of Italian grasspea (Lathyrus sativus L.) germplasm using agronomic traits, biochemical and molecular markers
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Characterization of Italian grasspea (Lathyrus sativus L.) germplasm using agronomic traits, biochemical and molecular markers

机译：利用农艺性状，生化和分子标记表征意大利草豌豆（Lathyrus sativus L.）种质

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Genetic relationships among 13 grasspea (Lathyrus sativus L.) landraces mainly collected in Southern Italy were assessed using agronomic traits, biochemical and molecular markers. Field trials were carried out in two locations and revealed a high influence of field locations on yield, but a low genotype × environment interaction. Despite this, the agronomic data obtained provided useful information for the choice of the best grasspea landraces for southern Italian marginal areas. Seed storage proteins utilised as biochemical markers were not able to detect polymorphisms, on the contrary both classes of molecular markers used i.e. AFLP and SSR, provided useful information on genetic variation and relationships among landraces. Even though the number of polymorphic fragments detected by AFLP technique was low, it was sufficient to discriminate all the accessions. The use of SSR to detect polymorphic sites in grasspea showed that most landraces were clearly grouped in two sub-clusters. One comprised two landraces from most northern localities, while all the other landraces were clustered together at a very narrow genetic distance.
机译：利用农艺性状，生化和分子标记，评估了13种主要在意大利南部收集的草豆（Lathyrus sativus L.）种的遗传关系。在两个地点进行了田间试验，结果表明田间地点对产量有很大影响，但基因型×环境的相互作用低。尽管如此，获得的农艺数据仍为选择意大利南部边缘地区的最佳草豌豆地方品种提供了有用的信息。用作生物化学标记的种子贮藏蛋白不能检测多态性，相反，使用的两类分子标记（即AFLP和SSR）都提供了遗传变异和地方物种之间关系的有用信息。即使通过AFLP技术检测到的多态性片段数量很少，也足以区分所有种质。利用SSR检测草豌豆中的多态性位点表明，大多数地方品种清楚地分为两个子类。其中一个包括来自大多数北部地区的两个地方种族，而所有其他地方种族则以很窄的遗传距离聚集在一起。

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