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首页> 外文期刊>Genetic Resources and Crop Evolution >Towards allelic diversity in the storage proteins of old and currently growing tetraploid and hexaploid wheats in Portugal
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Towards allelic diversity in the storage proteins of old and currently growing tetraploid and hexaploid wheats in Portugal

机译：寻求葡萄牙古老和目前正在生长的四倍体和六倍体小麦的贮藏蛋白中的等位基因多样性

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Using sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS–PAGE), the different alleles encoded at the 6 glutenin loci and 3 ω-gliadin loci were identified from a set of 134 hexaploid and 128 tetraploid wheat accessions mainly grown in Portugal. In the hexaploid wheats (T. aestivum L.), a total of 56, 42 and 36 patterns were observed for high molecular weight-glutenin subunits (HMW-GS), low molecular weight-glutenin subunits (LMW-GS) and ω-gliadins respectively. For HMW-GS encoded at Glu-A1, Glu-B1 and Glu-D1 loci, 4, 10 and 6 alleles were observed, respectively. LMW-GS displayed similar polymorphism, as Glu-A3, Glu-B3 and Glu-D3 loci, which comprises 5, 9 and 3 alleles. Twenty-four alleles were observed for ω-gliadins found at Gli-A1, Gli-B1 and Gli-D1 loci with, 5, 16 and 3 alleles respectively. For tetraploid collection fifty different alleles were identified for the seven loci studied Glu-A1 (3), Glu-B1 (13), Glu-A3 (6), Glu-B3 (7), Glu-B2 (2), Gli-A1 (5) and Gli-B1 (14). The genetic distances within hexaploid and tetraploid wheats were presented using cluster representation. The mean value of genetic variation indices (H) for wheat storage protein loci was slightly lower in current commercially available varieties (0.592) and highest for old varieties (0.574).
机译：使用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE），从主要在葡萄牙种植的134个六倍体和128个四倍体小麦种质中鉴定出6个谷蛋白基因座和3个ω-麦醇溶蛋白基因座编码的不同等位基因。在六倍体小麦（T. aestivum L.）中，共观察到56、42和36个模式的高分子量谷蛋白亚基（HMW-GS），低分子量谷蛋白亚基（LMW-GS）和ω-麦醇溶蛋白。对于在Glu-A1，Glu-B1和Glu-D1基因座编码的HMW-GS，分别观察到4、10和6个等位基因。 LMW-GS显示出相似的多态性，如Glu-A3，Glu-B3和Glu-D3位点，包括5、9和3个等位基因。在Gli-A1，Gli-B1和Gli-D1位点观察到的ω-麦醇溶蛋白有二十四个等位基因，分别具有5、16和3个等位基因。对于四倍体收集，为研究的七个基因座鉴定了五十个不同的等位基因Glu-A1（3），Glu-B1（13），Glu-A3（6），Glu-B3（7），Glu-B2（2），Gli- A1（5）和Gli-B1（14）。利用聚类表示法给出了六倍体和四倍体小麦内的遗传距离。小麦贮藏蛋白基因座的遗传变异指数（H）的平均值在目前的市售品种中略低（0.592），而在旧品种中最高（0.574）。

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