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首页> 外文期刊>Genetic Resources and Crop Evolution >Testing of rye-specific markers located on 1RS chromosome and distribution of 1AL.RS and 1BL.RS translocations in Turkish wheat (Triticumaestivum L., T.durum Desf.) varieties and landraces
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Testing of rye-specific markers located on 1RS chromosome and distribution of 1AL.RS and 1BL.RS translocations in Turkish wheat (Triticumaestivum L., T.durum Desf.) varieties and landraces

机译：在土耳其小麦（Triticumaestivum L.，T.durum Desf。）品种和地方品种中检测位于1RS染色体上的黑麦特异性标记以及1AL.RS和1BL.RS易位的分布

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摘要

Rye (Secale cereale L.) is a valuable source for alien chromosome translocations in wheat breeding, due to its capability to grow and sustain under harsh environmental conditions. Wheat germplasm with 1AL.1RS and 1BL.1RS wheat-rye chromosome translocations have been used worldwide by breeders. Determining 1AL.1RS and 1BL.1RS translocations in wheat is therefore of important practical value for wheat improvement. In this study, nine rye-specific markers detecting the rye chromosome 1RS in wheat background were evaluated. The markers PAWS5/S6, SCM9 and O-SEC5′-A/O-SEC3′-R amplified specific bands associated with 1AL.1RS and 1BL.1RS translocations. These three markers therefore provide a quick and reliable tool to identify and to discriminate these two wheat-rye translocations in wheat background. Six out of nine rye specific markers were subsequently used to determine the frequency of these translocations in commonly grown bread and durum wheat cultivars from Turkey. One hundred seven wheat cultivars and landraces were molecularly screened. Among them, only 4% (‘Seri-82’, ‘Yıldız-98’, ‘Tahirova’, and ‘Osmaniyem’) harbor the 1BL.1RS translocation whereas the 1AL.1RS translocation was not found. The information provided here will contribute to the creation of new Turkish wheat populations with a larger genetic diversity necessary for future requirements. Keywords 1RS translocation - Rye - Rye-specific markers - Secale cereale - Triticum - Turkey - Wheat - Wheat-rye translocation
机译：黑麦（Secale graine L.）是小麦育种中外来染色体易位的重要来源，因为它具有在恶劣环境条件下生长和维持的能力。育种家已在世界范围内使用具有1AL.1RS和1BL.1RS小麦-黑麦染色体易位的小麦种质。因此，确定小麦中的1AL.1RS和1BL.1RS易位对于改善小麦具有重要的实用价值。在这项研究中，评估了检测小麦背景中黑麦1RS染色体的9个黑麦特异性标记。标记PAWS5 / S6，SCM9和O-SEC5'-A / O-SEC3'-R扩增了与1AL.1RS和1BL.1RS易位相关的特定条带。因此，这三个标记提供了一种快速可靠的工具，可以识别和区分小麦背景中的这两个小麦-黑麦易位。随后，使用九个黑麦特异性标记物中的六个来确定来自土耳其的常见面包和硬质小麦品种中这些易位的频率。对一百七个小麦品种和地方品种进行了分子筛选。其中只有4％（“ Seri-82”，“Yıldız-98”，“ Tahirova”和“ Osmaniyem”）具有1BL.1RS易位，而没有发现1AL.1RS易位。此处提供的信息将有助于创建具有更大遗传多样性的土耳其新小麦种群，以满足未来需求。关键词1RS易位-黑麦-黑麦特异标记-麦片谷物-小麦-土耳其-小麦-小麦-黑麦易位

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