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Chromosomal regions controlling seedling drought resistance in Israeli wild barley, Hordeum spontaneum C. Koch

机译:控制以色列野生大麦(Cordeum spontaneum C. Koch)幼苗抗旱性的染色体区域

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摘要

The objective of the present study was to map quantitative trait loci (QTL) controlling drought-resistance traits at seedling stage of Israeli wild barley (Hordeum spontaneum). Mapping was conducted using an F4 mapping population derived from a cross between genotype WQ23-38 from xeric (Wadi Qilt, 144 mm annual rainfall) and MA10-30 from mesic (Maalot, 790 mm annual rainfall) environments. Obvious segregation was observed for traits including time to wilt, leaf relative elongation rate, recovery rate, shoot regrowth, osmolarity, relative water content, leaf length, and root length with the coefficient of variation ranging from 10 to 77%. Eighteen putative QTL effects were identified (at false discovery rate = 10%) by single-trait analyses and confirmed by multi-trait analysis and multiple interval mapping using a new MultiQTL package. The results of our study suggest that the xeric genotypes adopted survival strategies while the mesic genotypes adopted growth-sustain strategies to cope with drought stress. Keywords Drought resistance - Genetic map - Hordeum spontaneum - QTL mapping - Wild barley
机译:本研究的目的是绘制控制以色列野生大麦(Hordeum spontaneum)苗期抗旱性状的数量性状位点(QTL)。使用F 4 作图种群进行作图,该种群来自于干旱的WQ23-38基因型(Wadi Qilt,年降水量144毫米)和中等的MA10-30(Maalot,790毫米年降水量)环境。观察到明显的分离现象,包括枯萎时间,叶片相对伸长率,恢复率,枝条长势,渗透压,相对含水量,叶片长度和根长,变异系数在10%至77%之间。通过单性状分析鉴定出18个假定的QTL效应(错误发现率= 10%),并使用新的MultiQTL软件包通过多性状分析和多区间作图确认。我们的研究结果表明,干性基因型采取生存策略,而中型基因型采取生长维持策略来应对干旱胁迫。关键词抗旱性-遗传图谱-自然发芽大麦-QTL作图-野生大麦

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