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Genetic characterization and species relationships among selected Asiatic Vigna Savi

机译:亚洲部分Vi豆的遗传特征和物种关系

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Twenty-four ISSR primers were employed for diversity analysis, genotyping and assessment of species relationships in Asiatic Vigna from the Indian subcontinent. A total of 396 fragments were scored with an average of 16.5 products per primer. Primer no 827 was the most informative primer with highest resolving power (Rp), marker index (MI) as well as genotypic index (GI). Linear correlation was observed between Rp, MI and GI. The wild forms, V. mungo var. silvestris, V. radiata var. sublobata and V. radiata var. setulosa grouped closer to their respective cultigens. V. hainiana emerged as a distinct taxon and seems to be more primitive in comparison to the other close wild relatives of greengram and blackgram. Moderate gene flow and genetic differentiation was detected between the species analyzed. Analysis of molecular variance performed for the group (mungo–radiata–hainiana) in three different combinations revealed that nearly half of the variation exists within the populations while the remaining half is equally distributed among species and among populations within species.
机译:使用二十四份ISSR引物对印度次大陆的亚洲Vi豆进行了多样性分析,基因分型和物种关系评估。总共对396个片段进行评分,每个引物平均有16.5个产物。 827号引物是信息最丰富的引物,具有最高的分辨力(Rp),标记指数(MI)和基因型指数(GI)。在Rp,MI和GI之间观察到线性相关。野生形式,V。mungo var。 silvestris,V. radiata var。亚叶和V. radiata var。 setulosa靠近他们各自的品种。海茵弧菌(V. hainiana)作为一种独特的分类群出现,与格林格拉姆和黑格拉姆的其他近亲相比,似乎更为原始。在所分析的物种之间检测到适度的基因流和遗传分化。对三种不同组合(蒙哥-辐射-哈伊那亚州)进行的分子变异分析表明,近一半的变异存在于种群中,而其余的一半则在种间和种内种群之间平均分布。

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