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首页> 外文期刊>Genetic Resources and Crop Evolution >DNA markers and pollen morphology reveal that Praecitrullus fistulosus is more closely related to Benincasa hispida than to Citrullus spp.
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DNA markers and pollen morphology reveal that Praecitrullus fistulosus is more closely related to Benincasa hispida than to Citrullus spp.

机译：DNA标记和花粉形态揭示了Praecitrullus fistulosus与Benincasa hispida的关系比与Citrullus spp的关系更紧密。

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摘要

The round melon Praecitrullus fistulosus (Stocks) Pangalo has been cultivated in Asia since ancient times and has been considered an underexploited crop in the western world. In the USA, there is an increased interest in using P. fistulosus as a commercial vegetable, and possibly as a rootstock for grafting watermelon, melon, or cucumber. However, the taxonomic classification of P. fistulosus is incomplete and for many years it has been considered a close relative of watermelon [Citrullus lanatus subsp. vulgaris (Schrad. ex Eckl. et Zeyh.) Fursa] and was previously classified as Citrullus lanatus subsp. fistulosus (Stocks) Duthie et J.B. Fuller. Here, we used two sets of DNA markers to assess the genetic similarity of P. fistulosus in relation to Citrullus spp. {including Citrullus lanatus subsp. vulgaris, C. lanatus subsp. lanatus, Citroides group [also known as C. lanatus (Thunb.) Matsum. et Nakai subsp. lanatus var. citroides (Bailey) Mansf. ex Greb.], and C. colocynthis (L.) Schrad.}, Cucumis spp. (including C. melo, C. sativus, C. anguria, C. meeusei, C. zeyheri), Benincasa hispida (Thunb.) Cogn., Lagenaria siceraria (Mol.) Standl. and Cucurbita spp. (including C. moschata Duchesne and the winter squash C. maxima Duchesne). The first marker set comprised 501 markers that were produced by 38 primer pairs derived from watermelon expressed sequenced tags (ESTs) containing simple sequence repeat (SSR) motifs (designated as EST-SSR primers; produced 311 markers), and by 18 primer pairs derived from ESTs that do not contain SSR motives (designated here as EST-PCR primers; produced 190 markers). The second marker set comprised 628 markers that were produced by 18 sequence related amplified polymorphism (SRAP) primer pairs. The phylogenetic data indicated that among these cucurbit species, the wax gourd B. hispida is the closest to the P. fistulosus. Pollen observations, using light microscopy, indicated that each of the cucurbit genera examined here has unique pollen morphology. The Cucurbita spp. have globular pollen grains with a stigmatic surface. The L. siceraria has polygonal pollen grains with symmetrical boundaries, while the Citrullus spp. and Cucumis spp. have ovular (conical) and triangular shaped pollen grains (respectively). The B. hispida and P. fistulosus have spherical or semispherical pollen grains. These pollen features appear to be in agreement with the phylogenetic relationships of these two species based on DNA markers. Analysis with 12 SRAP primer pairs revealed low genetic diversity among 18 United States Plant Introductions (PIs) of P. fistulosus, indicating the need to expand the germplasm collection of this cucurbit crop.
机译：Pangalo圆瓜Praecitrullus fistulosus（Stocks）Pangalo自古以来就在亚洲栽培，在西方世界被认为是未开发的农作物。在美国，越来越多的兴趣将恶臭假单胞菌用作商品蔬菜，并有可能用作嫁接西瓜，甜瓜或黄瓜的砧木。然而，瘘管假单胞菌的分类学分类不完整，并且多年以来一直被认为是西瓜的近亲[Citrullus lanatus亚种。寻常型（Schrad。ex Eckl。et Zeyh。）Fursa]，以前被归类为柑桔亚种。瘘管（股票）Duthie等J.B.富勒。在这里，我们使用了两组DNA标记来评估假单胞菌与柑橘属的遗传相似性。 {包括柑桔属亚种。寻常型，C. lanatus子亚种cnatroides组的lanatus [也称为C. lanatus（Thunb。）Matsum。 et Nakai subsp。拉纳图斯山柠檬酸（Bailey）Mansf。 ]和C. colocynthis（L.）Schrad。}，Cucumis spp。 （包括C. melo，C。sativus，C。anguria，C。meeusei，C。zeyheri），Benincasa hispida（Thunb。）Cogn。，Lagenaria siceraria（Mol。）Standl。和南瓜属。 （包括C. moschata Duchesne和西葫芦C.maxima Duchesne）。第一组标记物包含501个标记物，这些标记物由38个引物对产生，引物对来自西瓜表达的带有简单序列重复（SSR）基序的表达标签（EST）（指定为EST-SSR引物；产生311个标记物），以及18个引物对来自不包含SSR动机的EST（此处指定为EST-PCR引物；产生190个标记）。第二标记集包括由18个序列相关的扩增多态性（SRAP）引物对产生的628个标记。系统发育数据表明，在这些葫芦科物种中，丝瓜B. hispida最接近于P. fistulosus。使用光学显微镜的花粉观察表明，这里检查的每个葫芦属都有独特的花粉形态。南瓜属。球状花粉粒表面带有散光。 L. siceraria具有多边形花粉粒，具有对称边界，而Citrullus spp。和黄瓜属。具有卵形（圆锥形）和三角形花粉颗粒（分别）。 B. hispida和P. fistulosus具有球形或半球形的花粉粒。这些花粉特征似乎与基于DNA标记的这两个物种的系统发育关系一致。用12对SRAP引物对进行的分析显示，美国18个P. fistulosus植物引种（PI）中的遗传多样性较低，这表明需要扩大这种葫芦科作物的种质资源。

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