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Development and utilization of diagnostic DAMD-PCR markers for Capsicum accessions

机译：辣椒种质的诊断性DAMD-PCR标记的开发和利用

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A polymerase chain reaction (PCR) based approach involving the directed amplification of minisatellite DNA region (DAMD-PCR) was used to identify accession specific DNA markers and study genetic relationships between and within 15 accessions corresponding to 11 species in genus Capsicum. A touch down PCR profile and unique chemical concentration of ingredients resulted in reproducible and reliable DNA amplifications. The number of amplified products varied from 1 to 12 fragments depending on the template DNA and the primers. The DAMD-PCR technique provided a total of 38 accession specific DNA markers (diagnostic DAMD-PCR) which can be utilized in accession identification, preservation and genetic studies of Capsicum germplasm. Based on 1,292 polymorphic and monomorphic DNA markers directed with 22 minisatellite specific primers, accessions were divided into four major groups, three of which corresponded to the three distinct Capsicum complexes. Capsicum chacoense was found to be the most distinct species.
机译：基于聚合酶链反应（PCR）的方法涉及到小卫星DNA区域的定向扩增（DAMD-PCR），用于鉴定特定于种质的DNA标记，并研究与辣椒属11种相对应的15种种质之间和之内的遗传关系。触底PCR谱图和成分的独特化学浓度可实现可重复且可靠的DNA扩增。取决于模板DNA和引物，扩增产物的数目从1至12个片段变化。 DAMD-PCR技术提供了总共38种登录特异性DNA标记（可诊断DAMD-PCR），可用于辣椒种子种质的登录鉴定，保存和遗传研究。基于针对22个微卫星特异性引物的1,292个多态性和单态性DNA标记，将种质分为四个主要组，其中三个对应于三个不同的辣椒属复合物。发现辣椒辣椒是最独特的物种。

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