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Characterization of European hazelnut ( Corylus avellana ) cultivars using SSR markers

机译:使用SSR标记表征欧洲榛子(榛子)品种

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摘要

World hazelnut production is based primarily on selections from the wild. In this study, we used 21 pairs of simple sequence repeat (SSR) primers to investigate genetic diversity in 270 clonal accessions of European hazelnut (Corylus avellana) representing a wide geographic range. Of the 270 accessions, 198 had unique fingerprints while 72 were duplicates. Based on the 198 unique accessions, the number of detected alleles per locus averaged 9.81 and observed heterozygosity (Ho) averaged 0.67. Of the 206 total alleles amplified, 20 were unique to a single accession. A genetic similarity matrix was constructed and the resulting dendrogram revealed four major geographical groups: Central European, Black Sea, English and Spanish-Italian. SSR alleles indicated the parentage of 31 accessions. The fingerprints are publicly available through the Germplasm Resources Information Network (GRIN) database. The identification of duplicate and mislabeled accessions will improve management of hazelnut genebanks, and information on genetic variation in hazelnut will assist the international research community.
机译:世界榛子的生产主要基于野外的选择。在这项研究中,我们使用了21对简单序列重复(SSR)引物来研究270个欧洲榛子(Corylus avellana)克隆克隆的遗传多样性,这些克隆代表了广泛的地理范围。在270个种质中,198个具有独特的指纹,而72个重复。基于这198个独特种质,每个基因座检测到的等位基因平均数为9.81,观察到的杂合度(H o )平均为0.67。在206个扩增的等位基因中,有20个是单个登录所独有的。构建了遗传相似性矩阵,结果树状图显示了四个主要地理区域:中欧,黑海,英语和西班牙语-意大利语。 SSR等位基因表明有31个种质。指纹可以通过种质资源信息网络(GRIN)数据库公开获得。鉴定重复和标签错误的登录品将改善榛子种质库的管理,有关榛子遗传变异的信息将有助于国际研究界。

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