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首页> 外文期刊>Genetic Resources and Crop Evolution >Assessing diversity in common bean (Phaseolus vulgaris L.) accessions at phenotype and molecular level: a preliminary approach
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Assessing diversity in common bean (Phaseolus vulgaris L.) accessions at phenotype and molecular level: a preliminary approach

机译：在表型和分子水平上评估普通豆（菜豆）种的多样性：一种初步方法

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摘要

Assessing diversity across and within crop varieties is relevant to improve the description of collections in genebanks and in on-farm conservation. The differences among and within several common bean (Phaseolus vulgaris L.) landraces, collected from and maintained ex situ in NE Italy, were initially studied using a set of 15 phenotypic descriptors. From a subgroup of five bean accessions, microsatellite genotyping was performed after the extraction of DNA from each of 38–40 individuals. One of the 15 phenotypical descriptors (plant architecture) was of no use. Only ten out of the 23 molecular markers did work, however sufficient to discriminate the accessions. The phenotypic descriptors identified a portion of the within-population variability. A few discrepancies were obtained when observations of phenotype descriptors were run independently in two alpine locations in NE Italy. The genetic approach carried out separately on DNA of about 40 individuals clarified the structure of the five accessions.
机译：评估作物品种之间和作物品种内部的多样性与改善对种质库和农场保护中收集的描述有关。最初使用一组15个表型描述符研究了从意大利东北部收集并异地保存的几种常见豆类（菜豆）之间的差异。从五个豆类的亚组中，从38-40个个体中提取DNA进行微卫星基因分型。 15个表型描述符（植物结构）之一没有用。 23种分子标记中只有10种起作用，但是足以区分这些种质。表型描述符识别了人口内部变异的一部分。当在意大利东北部的两个高山位置独立进行表型描述符的观察时，获得了一些差异。对约40个个体的DNA分别进行的遗传方法阐明了这5个种质的结构。

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